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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30553 | 5' | -57.7 | NC_006548.1 | + | 7569 | 0.66 | 0.446143 |
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Target: 5'- gGCCgaGCAGGCCGAggagcaagguGC-CGACCaucgcGCCUu -3' miRNA: 3'- -CGGa-CGUCCGGCU----------UGaGUUGG-----CGGAc -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 7520 | 0.66 | 0.466284 |
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Target: 5'- gGCCUGCucGGCC-AACUUGGCgaaGCCUu -3' miRNA: 3'- -CGGACGu-CCGGcUUGAGUUGg--CGGAc -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 7416 | 0.66 | 0.466284 |
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Target: 5'- aGCuCUGCAGGCagguCGAACUCGuugcguACCuCCUc -3' miRNA: 3'- -CG-GACGUCCG----GCUUGAGU------UGGcGGAc -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 7280 | 0.66 | 0.47653 |
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Target: 5'- -gCUGCcGGUCGAGgUCAACCagGCgCUGg -3' miRNA: 3'- cgGACGuCCGGCUUgAGUUGG--CG-GAC- -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 5724 | 0.68 | 0.336501 |
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Target: 5'- aGCCUGgAccaCGGACUCGAUgGCCUGg -3' miRNA: 3'- -CGGACgUccgGCUUGAGUUGgCGGAC- -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 5472 | 0.66 | 0.486886 |
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Target: 5'- cGCCggGCucAGGUCGAccGCUCgAGCCaggucgGCCUGg -3' miRNA: 3'- -CGGa-CG--UCCGGCU--UGAG-UUGG------CGGAC- -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 4882 | 0.68 | 0.353348 |
|
Target: 5'- uGCCUcCGGGCuCGAuUUCGGCCGCUc- -3' miRNA: 3'- -CGGAcGUCCG-GCUuGAGUUGGCGGac -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 1035 | 0.68 | 0.361992 |
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Target: 5'- gGCCUGCAGGaCGAugUCuAUCGgCa- -3' miRNA: 3'- -CGGACGUCCgGCUugAGuUGGCgGac -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 965 | 0.72 | 0.181411 |
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Target: 5'- aCCUGCgccAGGCUGAGCaguugcugacugaguUCAAgCGCCUGg -3' miRNA: 3'- cGGACG---UCCGGCUUG---------------AGUUgGCGGAC- -5' |
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| 30553 | 5' | -57.7 | NC_006548.1 | + | 476 | 0.67 | 0.426496 |
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Target: 5'- aGCgaGCuGGCCGAACU-GAUCGUCg- -3' miRNA: 3'- -CGgaCGuCCGGCUUGAgUUGGCGGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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