Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 30571 | 5' | -59.5 | NC_006548.1 | + | 12794 | 0.67 | 0.315752 |
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Target: 5'- -cGGCACCugagcuguauugcUCGCCAgUGCCGCGCg- -3' miRNA: 3'- ccCCGUGGu------------AGCGGUaGUGGCGCGac -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 8125 | 0.67 | 0.326753 |
|
Target: 5'- aGGGagGCCGUCGCCga-ACUGCGCc- -3' miRNA: 3'- cCCCg-UGGUAGCGGuagUGGCGCGac -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 28281 | 0.67 | 0.363154 |
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Target: 5'- aGGGGCugCGggCGUCGUCAacgucaccaaguucgUCGCGgaGg -3' miRNA: 3'- -CCCCGugGUa-GCGGUAGU---------------GGCGCgaC- -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 23375 | 0.67 | 0.350434 |
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Target: 5'- cGGGGUugucaccggguggACCAggGCCAUCGCU-CGCUa -3' miRNA: 3'- -CCCCG-------------UGGUagCGGUAGUGGcGCGAc -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 18634 | 0.66 | 0.368331 |
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Target: 5'- cGGGCuuCCA-CGCCA--GCUGCGCUc -3' miRNA: 3'- cCCCGu-GGUaGCGGUagUGGCGCGAc -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 36677 | 0.66 | 0.385949 |
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Target: 5'- -uGGCACCGuaUCGaCgCAUCcCCGCGCg- -3' miRNA: 3'- ccCCGUGGU--AGC-G-GUAGuGGCGCGac -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 20520 | 0.66 | 0.394964 |
|
Target: 5'- uGGGCACUggCaCCGugggugacugcUCGCCGCGCg- -3' miRNA: 3'- cCCCGUGGuaGcGGU-----------AGUGGCGCGac -5' |
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| 30571 | 5' | -59.5 | NC_006548.1 | + | 18723 | 0.66 | 0.394964 |
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Target: 5'- uGGGCAgCCcugcggcgguuGUCGCCAUggcuuucgCAUCGCGCa- -3' miRNA: 3'- cCCCGU-GG-----------UAGCGGUA--------GUGGCGCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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