Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30571 | 5' | -59.5 | NC_006548.1 | + | 21149 | 0.7 | 0.229258 |
Target: 5'- -cGGCGCCAUCGCCGccCGCCGUa--- -3' miRNA: 3'- ccCCGUGGUAGCGGUa-GUGGCGcgac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 11658 | 0.7 | 0.223328 |
Target: 5'- cGGGaGCAUCGUCauGUCAUCGCCGCa--- -3' miRNA: 3'- -CCC-CGUGGUAG--CGGUAGUGGCGcgac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 23846 | 0.7 | 0.223328 |
Target: 5'- --uGCACU-UCGCCAUCACCGuCGCg- -3' miRNA: 3'- cccCGUGGuAGCGGUAGUGGC-GCGac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 21396 | 0.71 | 0.175639 |
Target: 5'- uGGGCgucgGCCGUCaGCgCAUCACCGCGagUGa -3' miRNA: 3'- cCCCG----UGGUAG-CG-GUAGUGGCGCg-AC- -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 32410 | 0.72 | 0.145036 |
Target: 5'- -cGGCcaGCCGUCGCCGccguugccguUCGCgGCGCUGc -3' miRNA: 3'- ccCCG--UGGUAGCGGU----------AGUGgCGCGAC- -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 17516 | 0.75 | 0.100811 |
Target: 5'- cGGccaGCugCggCGCCAUCGCCGCuGCUGg -3' miRNA: 3'- -CCc--CGugGuaGCGGUAGUGGCG-CGAC- -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 29415 | 0.76 | 0.082596 |
Target: 5'- aGGGUGCCAUCGgCGagACCGCGCa- -3' miRNA: 3'- cCCCGUGGUAGCgGUagUGGCGCGac -5' |
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30571 | 5' | -59.5 | NC_006548.1 | + | 36134 | 1.11 | 0.000178 |
Target: 5'- aGGGGCACCAUCGCCAUCACCGCGCUGg -3' miRNA: 3'- -CCCCGUGGUAGCGGUAGUGGCGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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