Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30584 | 5' | -51.3 | NC_006549.1 | + | 125231 | 0.66 | 0.988146 |
Target: 5'- uGCGgAGAcUGCCCcguUACGAuccAUGCACa -3' miRNA: 3'- uCGCgUUUuGCGGG---AUGCUu--UGCGUG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 64224 | 0.66 | 0.988146 |
Target: 5'- -aUGCcgGAGACGCCCgaaaugUACGcAGCGUACg -3' miRNA: 3'- ucGCG--UUUUGCGGG------AUGCuUUGCGUG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 130616 | 0.66 | 0.986537 |
Target: 5'- uGGUGuCGGAACGUCUggaAgGAGugGCGCu -3' miRNA: 3'- -UCGC-GUUUUGCGGGa--UgCUUugCGUG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 120096 | 0.66 | 0.986367 |
Target: 5'- cGGCGgucuaaaCGGAuucGCGCCCgaauuUGCGuuACGCGCg -3' miRNA: 3'- -UCGC-------GUUU---UGCGGG-----AUGCuuUGCGUG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 86173 | 0.66 | 0.98495 |
Target: 5'- gAGCGUAcGACGCCUcacaccaaucgcccgACGAGGgGCAg -3' miRNA: 3'- -UCGCGUuUUGCGGGa--------------UGCUUUgCGUg -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 20119 | 0.66 | 0.984765 |
Target: 5'- -aCGgAAAcCGCCCU-CGAaGACGCGCu -3' miRNA: 3'- ucGCgUUUuGCGGGAuGCU-UUGCGUG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 73256 | 0.66 | 0.984765 |
Target: 5'- gGGCGCAucagacACGCCUUuuCG-GACGCAa -3' miRNA: 3'- -UCGCGUuu----UGCGGGAu-GCuUUGCGUg -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 96461 | 0.66 | 0.984765 |
Target: 5'- cGCGCAAuccauaGGCGCCaaAC-AGACGCAg -3' miRNA: 3'- uCGCGUU------UUGCGGgaUGcUUUGCGUg -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 90187 | 0.66 | 0.982822 |
Target: 5'- -aCGaAAAGCGCCCUAagaGGAugGCGa -3' miRNA: 3'- ucGCgUUUUGCGGGAUg--CUUugCGUg -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 60475 | 0.66 | 0.982822 |
Target: 5'- cGCcCGAGGCGCCC-GCGAcGGCGUugGCg -3' miRNA: 3'- uCGcGUUUUGCGGGaUGCU-UUGCG--UG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 126934 | 0.66 | 0.982822 |
Target: 5'- uGGCGUGAAauacACGCCCgaaGAcgUGUACg -3' miRNA: 3'- -UCGCGUUU----UGCGGGaugCUuuGCGUG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 57017 | 0.66 | 0.982822 |
Target: 5'- cGCGCcuGACGCCacaaGAGAC-CACa -3' miRNA: 3'- uCGCGuuUUGCGGgaugCUUUGcGUG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 36715 | 0.66 | 0.982822 |
Target: 5'- gAGaCGCAgaaccccgcAGACGUCCUcucacgucaaACGAAAUGUGCg -3' miRNA: 3'- -UC-GCGU---------UUUGCGGGA----------UGCUUUGCGUG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 73349 | 0.66 | 0.980697 |
Target: 5'- cGCGCcuuGCGCUCUgagucauccacACGuuACGUACg -3' miRNA: 3'- uCGCGuuuUGCGGGA-----------UGCuuUGCGUG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 72049 | 0.67 | 0.978382 |
Target: 5'- -cUGCGAAACcCCCgcCGAAcccACGCACg -3' miRNA: 3'- ucGCGUUUUGcGGGauGCUU---UGCGUG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 10448 | 0.67 | 0.975868 |
Target: 5'- cGCGCGgaAGACGCUCUGagaAAACGC-Ca -3' miRNA: 3'- uCGCGU--UUUGCGGGAUgc-UUUGCGuG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 47949 | 0.67 | 0.973146 |
Target: 5'- cAGCGCAAAACGUgCUGgaAGACGgAUc -3' miRNA: 3'- -UCGCGUUUUGCGgGAUgcUUUGCgUG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 30080 | 0.67 | 0.973146 |
Target: 5'- uAG-GCAAAGCGCgCaa-GAAGCGUACg -3' miRNA: 3'- -UCgCGUUUUGCGgGaugCUUUGCGUG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 30950 | 0.67 | 0.970209 |
Target: 5'- uAGCGCAcaauauaacgcAAACGUCCguggaagGCGgcAUGUACa -3' miRNA: 3'- -UCGCGU-----------UUUGCGGGa------UGCuuUGCGUG- -5' |
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30584 | 5' | -51.3 | NC_006549.1 | + | 23854 | 0.67 | 0.970209 |
Target: 5'- cGGCGUAu--UGCCagaaUACGAAaacgacACGCGCg -3' miRNA: 3'- -UCGCGUuuuGCGGg---AUGCUU------UGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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