Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30688 | 5' | -59.2 | NC_006552.1 | + | 23674 | 0.71 | 0.290247 |
Target: 5'- gGGCUGC-AUGGUGcugcuACCaCUCGcCGGCAu -3' miRNA: 3'- -CCGGCGuUGCCACu----UGG-GAGC-GCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 57177 | 0.7 | 0.326841 |
Target: 5'- aGCgCGaCAGCGGUGcauAGCCCUCGCccuGCAa -3' miRNA: 3'- cCG-GC-GUUGCCAC---UUGGGAGCGc--CGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 7689 | 0.69 | 0.39225 |
Target: 5'- gGGCCGCc-CGGUGAA----CGCGGCAa -3' miRNA: 3'- -CCGGCGuuGCCACUUgggaGCGCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 20398 | 0.68 | 0.417973 |
Target: 5'- aGCgGCAACcGUGAaucguccGCCCUCcCGGCGa -3' miRNA: 3'- cCGgCGUUGcCACU-------UGGGAGcGCCGU- -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 21318 | 0.68 | 0.427996 |
Target: 5'- uGGCC-CAuGCGGUGGACCag-GCGGUg -3' miRNA: 3'- -CCGGcGU-UGCCACUUGGgagCGCCGu -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 45636 | 0.68 | 0.427996 |
Target: 5'- cGGCCGCuagcugAGCGGUGuacugccccagGGCCUUCGCcGCc -3' miRNA: 3'- -CCGGCG------UUGCCAC-----------UUGGGAGCGcCGu -5' |
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30688 | 5' | -59.2 | NC_006552.1 | + | 43330 | 0.66 | 0.587044 |
Target: 5'- cGCCGCcaGAUGGUccAGCaCCagCGCGGCAa -3' miRNA: 3'- cCGGCG--UUGCCAc-UUG-GGa-GCGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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