Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30695 | 3' | -62.9 | NC_006552.1 | + | 53618 | 0.66 | 0.37241 |
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Target: 5'- uGCCACUGCgCCGGCccagGGCCuGCAc -3' miRNA: 3'- gCGGUGGCGgGGUCGccuaCUGG-CGU- -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 56050 | 0.66 | 0.380703 |
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Target: 5'- uGCCGCCGUcaCCCuGCGcGUaGCCGCu -3' miRNA: 3'- gCGGUGGCG--GGGuCGCcUAcUGGCGu -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 56733 | 0.7 | 0.216363 |
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Target: 5'- uGCC-CUGCUUCAGCaGGAUGuCCGCc -3' miRNA: 3'- gCGGuGGCGGGGUCG-CCUACuGGCGu -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 57854 | 0.66 | 0.355399 |
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Target: 5'- gCGCCAucugucgugcugcCCGCCCCAGUucc--GCCGCAg -3' miRNA: 3'- -GCGGU-------------GGCGGGGUCGccuacUGGCGU- -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 59759 | 0.71 | 0.172411 |
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Target: 5'- gCGCCaguuguucGCCGCgCUGGCGGGUGAUCgGCGg -3' miRNA: 3'- -GCGG--------UGGCGgGGUCGCCUACUGG-CGU- -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 59888 | 0.66 | 0.380703 |
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Target: 5'- aGCUACCGCgCCAGuuGGUGauGCCGa- -3' miRNA: 3'- gCGGUGGCGgGGUCgcCUAC--UGGCgu -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 60409 | 0.66 | 0.371587 |
|
Target: 5'- gCGCCACCacuacaGCCCCcaccugcGGCGGGggcGAUgCGCAc -3' miRNA: 3'- -GCGGUGG------CGGGG-------UCGCCUa--CUG-GCGU- -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 61610 | 0.68 | 0.276087 |
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Target: 5'- aGCCGCCGgCgCCGGCaGGAcuuGCCGCu -3' miRNA: 3'- gCGGUGGCgG-GGUCG-CCUac-UGGCGu -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 63523 | 0.68 | 0.303436 |
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Target: 5'- cCGCCGCCGCCagcaCgAGCGugucacacGAUGGCuCGCu -3' miRNA: 3'- -GCGGUGGCGGg---G-UCGC--------CUACUG-GCGu -5' |
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| 30695 | 3' | -62.9 | NC_006552.1 | + | 64221 | 0.68 | 0.289508 |
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Target: 5'- cCGCUcgauGCUGCCCCcGCGGAUGAUa--- -3' miRNA: 3'- -GCGG----UGGCGGGGuCGCCUACUGgcgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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