Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30791 | 3' | -64.8 | NC_006552.1 | + | 7190 | 0.72 | 0.115049 |
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Target: 5'- aUCaCCCAagcgcGUCCCGuCCCCGGCGCgCAGc -3' miRNA: 3'- -AGcGGGU-----CAGGGC-GGGGUCGCG-GUCc -5' |
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| 30791 | 3' | -64.8 | NC_006552.1 | + | 51167 | 0.72 | 0.115049 |
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Target: 5'- gUCGCCgAGaaggCCCGCgCCaaAGCGCCAGu -3' miRNA: 3'- -AGCGGgUCa---GGGCGgGG--UCGCGGUCc -5' |
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| 30791 | 3' | -64.8 | NC_006552.1 | + | 30563 | 0.72 | 0.115049 |
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Target: 5'- -gGCCCcG-CCgGUCCaCAGCGCCGGGa -3' miRNA: 3'- agCGGGuCaGGgCGGG-GUCGCGGUCC- -5' |
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| 30791 | 3' | -64.8 | NC_006552.1 | + | 48226 | 0.73 | 0.103089 |
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Target: 5'- -gGCCCugcuaagcaaCCCGCCCCGGUucGCCGGGa -3' miRNA: 3'- agCGGGuca-------GGGCGGGGUCG--CGGUCC- -5' |
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| 30791 | 3' | -64.8 | NC_006552.1 | + | 7361 | 0.74 | 0.090856 |
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Target: 5'- cCGcCCCAGUUCCGCCgCAGCGCg--- -3' miRNA: 3'- aGC-GGGUCAGGGCGGgGUCGCGgucc -5' |
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| 30791 | 3' | -64.8 | NC_006552.1 | + | 16435 | 1.09 | 0.000177 |
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Target: 5'- cUCGCCCAGUCCCGCCCCAGCGCCAGGu -3' miRNA: 3'- -AGCGGGUCAGGGCGGGGUCGCGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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