Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30878 | 3' | -59.2 | NC_006552.1 | + | 54283 | 0.69 | 0.41506 |
Target: 5'- aCCaC-CUACGGCuUCGcGCCGCCGGu -3' miRNA: 3'- -GGcGcGAUGUCGuAGCuCGGCGGCCu -5' |
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30878 | 3' | -59.2 | NC_006552.1 | + | 59579 | 0.66 | 0.561431 |
Target: 5'- aUGC-CUACGGCGUgGAGCUGgacCCGGc -3' miRNA: 3'- gGCGcGAUGUCGUAgCUCGGC---GGCCu -5' |
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30878 | 3' | -59.2 | NC_006552.1 | + | 60279 | 0.66 | 0.5812 |
Target: 5'- gCCGCGCcACccggcuggcccugGGCAUCG-GCCcCUGGGu -3' miRNA: 3'- -GGCGCGaUG-------------UCGUAGCuCGGcGGCCU- -5' |
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30878 | 3' | -59.2 | NC_006552.1 | + | 61440 | 0.7 | 0.347131 |
Target: 5'- cCCGCGCccgggccgUACGGCAauaCGAG-UGCCGGGa -3' miRNA: 3'- -GGCGCG--------AUGUCGUa--GCUCgGCGGCCU- -5' |
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30878 | 3' | -59.2 | NC_006552.1 | + | 62759 | 0.67 | 0.510419 |
Target: 5'- -gGCGCUGCcGCGccugCGAGCgaCGCCuGGAc -3' miRNA: 3'- ggCGCGAUGuCGUa---GCUCG--GCGG-CCU- -5' |
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30878 | 3' | -59.2 | NC_006552.1 | + | 63191 | 0.66 | 0.551099 |
Target: 5'- -aGCGCUGCaaGGCGUUGuccuGGCCGCaaCGGu -3' miRNA: 3'- ggCGCGAUG--UCGUAGC----UCGGCG--GCCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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