Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30878 | 3' | -59.2 | NC_006552.1 | + | 33748 | 0.66 | 0.551099 |
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Target: 5'- gCCaGCGCggcgGCGGCAuugcugUUGAGCagaaCGCCGGc -3' miRNA: 3'- -GG-CGCGa---UGUCGU------AGCUCG----GCGGCCu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 59579 | 0.66 | 0.561431 |
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Target: 5'- aUGC-CUACGGCGUgGAGCUGgacCCGGc -3' miRNA: 3'- gGCGcGAUGUCGUAgCUCGGC---GGCCu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 12210 | 0.66 | 0.571815 |
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Target: 5'- aCCGCGCagaggccuCAGCG-CGGGCgGCCa-- -3' miRNA: 3'- -GGCGCGau------GUCGUaGCUCGgCGGccu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 60279 | 0.66 | 0.5812 |
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Target: 5'- gCCGCGCcACccggcuggcccugGGCAUCG-GCCcCUGGGu -3' miRNA: 3'- -GGCGCGaUG-------------UCGUAGCuCGGcGGCCU- -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 9656 | 0.66 | 0.582245 |
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Target: 5'- uUGCGCUcucGCcuGCAucaaucauUCGAGCCGCCa-- -3' miRNA: 3'- gGCGCGA---UGu-CGU--------AGCUCGGCGGccu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 37776 | 0.66 | 0.591664 |
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Target: 5'- cCCGCGCUcugcaucACAGCGUCGGcGaUCGgCGcGAu -3' miRNA: 3'- -GGCGCGA-------UGUCGUAGCU-C-GGCgGC-CU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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