Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30878 | 3' | -59.2 | NC_006552.1 | + | 32347 | 0.69 | 0.375008 |
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Target: 5'- gCCGCGCacgGCGGCuucgcgcacugcgucGUUGAGCCaGUCGGc -3' miRNA: 3'- -GGCGCGa--UGUCG---------------UAGCUCGG-CGGCCu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 32499 | 0.7 | 0.363348 |
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Target: 5'- gCGCGCguggACGGCAUCacggGAGCgCGCCa-- -3' miRNA: 3'- gGCGCGa---UGUCGUAG----CUCG-GCGGccu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 61440 | 0.7 | 0.347131 |
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Target: 5'- cCCGCGCccgggccgUACGGCAauaCGAG-UGCCGGGa -3' miRNA: 3'- -GGCGCG--------AUGUCGUa--GCUCgGCGGCCU- -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 43960 | 0.7 | 0.323792 |
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Target: 5'- cCCGCGCUccuuCAGCAggaacuugUCGAuGCCGCUGu- -3' miRNA: 3'- -GGCGCGAu---GUCGU--------AGCU-CGGCGGCcu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 37033 | 0.71 | 0.279999 |
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Target: 5'- aCGCGCUGCcaccgcuAGCGUCcuGAGagGCCGGAa -3' miRNA: 3'- gGCGCGAUG-------UCGUAG--CUCggCGGCCU- -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 10841 | 0.74 | 0.178545 |
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Target: 5'- gCCGgGCUGCGGCGcugccgacucuUCG-GCUGCCGGc -3' miRNA: 3'- -GGCgCGAUGUCGU-----------AGCuCGGCGGCCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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