Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 30878 | 3' | -59.2 | NC_006552.1 | + | 26289 | 0.69 | 0.406131 |
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Target: 5'- gCCGCGCc-CAGCGgguAGUCGCCGGc -3' miRNA: 3'- -GGCGCGauGUCGUagcUCGGCGGCCu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 54283 | 0.69 | 0.41506 |
|
Target: 5'- aCCaC-CUACGGCuUCGcGCCGCCGGu -3' miRNA: 3'- -GGcGcGAUGUCGuAGCuCGGCGGCCu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 13237 | 0.68 | 0.433274 |
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Target: 5'- gCGCGCUGCAugagggcuacauGCucAUCGAGC-GUCGGGg -3' miRNA: 3'- gGCGCGAUGU------------CG--UAGCUCGgCGGCCU- -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 38263 | 0.68 | 0.460488 |
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Target: 5'- aUCGCGCUGCGGCucGUUGAGCaccacgacacgauCGgCGGu -3' miRNA: 3'- -GGCGCGAUGUCG--UAGCUCG-------------GCgGCCu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 2038 | 0.68 | 0.461443 |
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Target: 5'- uCUGCGCcugggACAgGCGcUUGAGCgCGCCGGc -3' miRNA: 3'- -GGCGCGa----UGU-CGU-AGCUCG-GCGGCCu -5' |
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| 30878 | 3' | -59.2 | NC_006552.1 | + | 37776 | 0.66 | 0.591664 |
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Target: 5'- cCCGCGCUcugcaucACAGCGUCGGcGaUCGgCGcGAu -3' miRNA: 3'- -GGCGCGA-------UGUCGUAGCU-C-GGCgGC-CU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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