Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30883 | 5' | -53.7 | NC_006552.1 | + | 36166 | 0.67 | 0.782375 |
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Target: 5'- aUCGGCGC-UGGCC-UCGccaGCAucGCCGGc -3' miRNA: 3'- -GGUCGCGuAUUGGaAGCa--CGU--CGGCC- -5' |
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| 30883 | 5' | -53.7 | NC_006552.1 | + | 9157 | 0.67 | 0.801768 |
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Target: 5'- cCCuGCGCGUAGCCgcUCGcgGCAuCCGc -3' miRNA: 3'- -GGuCGCGUAUUGGa-AGCa-CGUcGGCc -5' |
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| 30883 | 5' | -53.7 | NC_006552.1 | + | 7691 | 0.67 | 0.801768 |
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Target: 5'- aCCAGCG-AUGcCCUUUcgGUGaauaAGCCGGc -3' miRNA: 3'- -GGUCGCgUAUuGGAAG--CACg---UCGGCC- -5' |
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| 30883 | 5' | -53.7 | NC_006552.1 | + | 54555 | 0.67 | 0.801768 |
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Target: 5'- gCCGGCGCAaGGCCUggugaUCGUGgAcaccGCCa- -3' miRNA: 3'- -GGUCGCGUaUUGGA-----AGCACgU----CGGcc -5' |
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| 30883 | 5' | -53.7 | NC_006552.1 | + | 52948 | 0.67 | 0.801768 |
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Target: 5'- gCCGGCGCcc-GCCgccgGUGCGGCaucCGGg -3' miRNA: 3'- -GGUCGCGuauUGGaag-CACGUCG---GCC- -5' |
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| 30883 | 5' | -53.7 | NC_006552.1 | + | 11334 | 0.67 | 0.801768 |
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Target: 5'- gCGGCGUcgAUGGCUUccUCGgugacgGCGGCCGa -3' miRNA: 3'- gGUCGCG--UAUUGGA--AGCa-----CGUCGGCc -5' |
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| 30883 | 5' | -53.7 | NC_006552.1 | + | 10921 | 0.66 | 0.830416 |
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Target: 5'- gUAGCGCuggguccauaaacgAACCUUcCG-GCAGCCGu -3' miRNA: 3'- gGUCGCGua------------UUGGAA-GCaCGUCGGCc -5' |
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| 30883 | 5' | -53.7 | NC_006552.1 | + | 37970 | 0.66 | 0.846162 |
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Target: 5'- gCCAGgGCGUcuugcacGGCCUggUCGaucUGCuGCUGGa -3' miRNA: 3'- -GGUCgCGUA-------UUGGA--AGC---ACGuCGGCC- -5' |
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| 30883 | 5' | -53.7 | NC_006552.1 | + | 35984 | 0.66 | 0.863623 |
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Target: 5'- aCCAGCuucuCGUAGCCgauggUGUGCuuGCCGu -3' miRNA: 3'- -GGUCGc---GUAUUGGaa---GCACGu-CGGCc -5' |
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| 30883 | 5' | -53.7 | NC_006552.1 | + | 60057 | 0.66 | 0.863623 |
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Target: 5'- gCuGCGCucgaGGCCUgCG-GCGGUCGGg -3' miRNA: 3'- gGuCGCGua--UUGGAaGCaCGUCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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