Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30924 | 3' | -56.2 | NC_006552.1 | + | 32696 | 0.66 | 0.715728 |
Target: 5'- uGUCGUCGccGGCCGGCGcgggGCCgGUGGa -3' miRNA: 3'- cUAGUAGU--CCGGUCGUag--CGG-CACCg -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 26706 | 0.66 | 0.705091 |
Target: 5'- uGGUCGcccaGGGCgccgaaGGCGUCGCCGUgcagugGGCu -3' miRNA: 3'- -CUAGUag--UCCGg-----UCGUAGCGGCA------CCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 6530 | 0.66 | 0.673889 |
Target: 5'- --cCAUCGGGCCAGCuauuuccaggacgucUCGCguaUGGCu -3' miRNA: 3'- cuaGUAGUCCGGUCGu--------------AGCGgc-ACCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 24720 | 0.66 | 0.661954 |
Target: 5'- gGGUCGU-AGGCCAcCAgccggCGCCG-GGCc -3' miRNA: 3'- -CUAGUAgUCCGGUcGUa----GCGGCaCCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 47673 | 0.66 | 0.661954 |
Target: 5'- gGAUC-UCAGGUCccccgagaaAGCAuUCGCCcagccggagGUGGCg -3' miRNA: 3'- -CUAGuAGUCCGG---------UCGU-AGCGG---------CACCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 53987 | 0.67 | 0.651076 |
Target: 5'- --gCAggCAGGCCAggcGCGUgGCCcUGGCc -3' miRNA: 3'- cuaGUa-GUCCGGU---CGUAgCGGcACCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 34926 | 0.67 | 0.651076 |
Target: 5'- aAUC-UCAGGCCAGUGcccUUGCCcucGGCg -3' miRNA: 3'- cUAGuAGUCCGGUCGU---AGCGGca-CCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 26341 | 0.67 | 0.628187 |
Target: 5'- --gCGUCAGGUagaGGCGcgCGCCGgucacacUGGCa -3' miRNA: 3'- cuaGUAGUCCGg--UCGUa-GCGGC-------ACCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 36342 | 0.67 | 0.618377 |
Target: 5'- --cUAUCccGCCAcaUAUCGCCGUGGCc -3' miRNA: 3'- cuaGUAGucCGGUc-GUAGCGGCACCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 51521 | 0.67 | 0.607488 |
Target: 5'- cAUCGaggCAGGCCAGauCAUCgacGCCGaGGCg -3' miRNA: 3'- cUAGUa--GUCCGGUC--GUAG---CGGCaCCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 45345 | 0.67 | 0.59662 |
Target: 5'- gGGUCuuUCGGGCCuGGCAugUCGgCCGguaucUGGCa -3' miRNA: 3'- -CUAGu-AGUCCGG-UCGU--AGC-GGC-----ACCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 35869 | 0.68 | 0.585782 |
Target: 5'- --aCGUCGGgGCCucGCc-UGCCGUGGCg -3' miRNA: 3'- cuaGUAGUC-CGGu-CGuaGCGGCACCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 55676 | 0.68 | 0.574982 |
Target: 5'- -uUCAUCGGcuGCCAGgAaggggucgaUCGCCG-GGCg -3' miRNA: 3'- cuAGUAGUC--CGGUCgU---------AGCGGCaCCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 63816 | 0.68 | 0.574982 |
Target: 5'- -uUCGgcgccCAGGCCAGCca-GCCGccGGCg -3' miRNA: 3'- cuAGUa----GUCCGGUCGuagCGGCa-CCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 39768 | 0.68 | 0.553528 |
Target: 5'- -uUCGUCcacGGUCAGCggcguGUCGCCcgcagGUGGCa -3' miRNA: 3'- cuAGUAGu--CCGGUCG-----UAGCGG-----CACCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 44364 | 0.69 | 0.532318 |
Target: 5'- -uUCGUCAGuGCCAGCA--GCCGgucggagGGUc -3' miRNA: 3'- cuAGUAGUC-CGGUCGUagCGGCa------CCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 41565 | 0.69 | 0.511407 |
Target: 5'- gGGUCAUCGGGUaccggcuugaaGGCAUUGCCacUGGUa -3' miRNA: 3'- -CUAGUAGUCCGg----------UCGUAGCGGc-ACCG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 6836 | 0.69 | 0.511407 |
Target: 5'- cGAUCAUCAGGgCAGCAgccuucCGaCCGUccuuGCg -3' miRNA: 3'- -CUAGUAGUCCgGUCGUa-----GC-GGCAc---CG- -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 23989 | 0.69 | 0.511407 |
Target: 5'- --gCAUCAGGCCGGUGUCGUCa---- -3' miRNA: 3'- cuaGUAGUCCGGUCGUAGCGGcaccg -5' |
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30924 | 3' | -56.2 | NC_006552.1 | + | 5425 | 0.69 | 0.480709 |
Target: 5'- --cCAUCAGGCUGcGCGccaguuguUCGCCGcgcUGGCg -3' miRNA: 3'- cuaGUAGUCCGGU-CGU--------AGCGGC---ACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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