Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31012 | 5' | -57.9 | NC_006560.1 | + | 4414 | 0.66 | 0.76605 |
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Target: 5'- cGUCGCCCAGCuCGgGCGCccacACGGGc- -3' miRNA: 3'- -UAGUGGGUCGcGCaCGCGa---UGCUCaa -5' |
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| 31012 | 5' | -57.9 | NC_006560.1 | + | 92891 | 0.66 | 0.76605 |
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Target: 5'- -gCGCCCuggAGCGCG-GCGgCgGCGAGUg -3' miRNA: 3'- uaGUGGG---UCGCGCaCGC-GaUGCUCAa -5' |
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| 31012 | 5' | -57.9 | NC_006560.1 | + | 39442 | 0.66 | 0.784869 |
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Target: 5'- uUCA-CCAGCGCcacgGCGCUGCGcGGUc -3' miRNA: 3'- uAGUgGGUCGCGca--CGCGAUGC-UCAa -5' |
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| 31012 | 5' | -57.9 | NC_006560.1 | + | 130701 | 0.66 | 0.784869 |
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Target: 5'- -aCACCgggaCGGCGCGguucGCGC-GCGAGUa -3' miRNA: 3'- uaGUGG----GUCGCGCa---CGCGaUGCUCAa -5' |
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| 31012 | 5' | -57.9 | NC_006560.1 | + | 87147 | 0.66 | 0.794074 |
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Target: 5'- gAUCACCCcGCGC-UGCGCgggcccgGCGGcGUa -3' miRNA: 3'- -UAGUGGGuCGCGcACGCGa------UGCU-CAa -5' |
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| 31012 | 5' | -57.9 | NC_006560.1 | + | 113646 | 0.66 | 0.80313 |
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Target: 5'- -gCGCCCGGCuuccccGCG-GgGCUGCGGGa- -3' miRNA: 3'- uaGUGGGUCG------CGCaCgCGAUGCUCaa -5' |
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| 31012 | 5' | -57.9 | NC_006560.1 | + | 132086 | 0.66 | 0.80313 |
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Target: 5'- cUCGCgCCGcgucguGCGCGUGUGCgGCGGGc- -3' miRNA: 3'- uAGUG-GGU------CGCGCACGCGaUGCUCaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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