Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31317 | 5' | -53.6 | NC_006623.1 | + | 113006 | 0.72 | 0.699609 |
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Target: 5'- gAGAAGUUucGCGGCGGCGGUugcGCgCGGu -3' miRNA: 3'- -UCUUUAA--CGUCGCCGCCGugaUG-GCC- -5' |
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| 31317 | 5' | -53.6 | NC_006623.1 | + | 111834 | 0.66 | 0.949187 |
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Target: 5'- uGAAAUgGCAGCGGCccGGCGggGCaUGGc -3' miRNA: 3'- uCUUUAaCGUCGCCG--CCGUgaUG-GCC- -5' |
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| 31317 | 5' | -53.6 | NC_006623.1 | + | 105793 | 0.66 | 0.960997 |
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Target: 5'- uAGAGuUUGCGGCaa-GGCA-UGCCGGa -3' miRNA: 3'- -UCUUuAACGUCGccgCCGUgAUGGCC- -5' |
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| 31317 | 5' | -53.6 | NC_006623.1 | + | 87196 | 0.71 | 0.794717 |
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Target: 5'- -cAGGUUGCAGCagagguuucgaaaaGGCGGCACcACCa- -3' miRNA: 3'- ucUUUAACGUCG--------------CCGCCGUGaUGGcc -5' |
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| 31317 | 5' | -53.6 | NC_006623.1 | + | 80857 | 0.66 | 0.967723 |
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Target: 5'- uGAGAUUGCuccuGCGGuCGGaGCc-CCGGa -3' miRNA: 3'- uCUUUAACGu---CGCC-GCCgUGauGGCC- -5' |
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| 31317 | 5' | -53.6 | NC_006623.1 | + | 66553 | 0.66 | 0.949187 |
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Target: 5'- uGAGuuUUGUAGCuGCGGCcggcgcggcucACUACCGa -3' miRNA: 3'- uCUUu-AACGUCGcCGCCG-----------UGAUGGCc -5' |
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| 31317 | 5' | -53.6 | NC_006623.1 | + | 39589 | 0.7 | 0.810156 |
|
Target: 5'- uGGGAUaGCGGUGGUGGUgagcauuauuuuaggACUGCUGGc -3' miRNA: 3'- uCUUUAaCGUCGCCGCCG---------------UGAUGGCC- -5' |
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| 31317 | 5' | -53.6 | NC_006623.1 | + | 27054 | 1.1 | 0.003909 |
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Target: 5'- gAGAAAUUGCAGCGGCGGCACUACCGGc -3' miRNA: 3'- -UCUUUAACGUCGCCGCCGUGAUGGCC- -5' |
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| 31317 | 5' | -53.6 | NC_006623.1 | + | 25712 | 0.66 | 0.967723 |
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Target: 5'- uAGAAGcgGUGGCGGgGGCGCgcaaauCCa- -3' miRNA: 3'- -UCUUUaaCGUCGCCgCCGUGau----GGcc -5' |
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| 31317 | 5' | -53.6 | NC_006623.1 | + | 25196 | 0.69 | 0.880008 |
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Target: 5'- -uAGGUUGUGG-GGCGGCACauCCGGc -3' miRNA: 3'- ucUUUAACGUCgCCGCCGUGauGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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