Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31390 | 3' | -57.4 | NC_006623.1 | + | 143655 | 0.74 | 0.456237 |
Target: 5'- uCC-CAGACCUAGGCCGCgGCaUUaCCCu -3' miRNA: 3'- -GGaGUUUGGAUCCGGCG-CGcAGcGGG- -5' |
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31390 | 3' | -57.4 | NC_006623.1 | + | 144472 | 0.68 | 0.785563 |
Target: 5'- --aUAAACCcc-GCCaaGCGUCGCCCg -3' miRNA: 3'- ggaGUUUGGaucCGGcgCGCAGCGGG- -5' |
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31390 | 3' | -57.4 | NC_006623.1 | + | 145724 | 0.67 | 0.836909 |
Target: 5'- gUCUCAGaacguGCCccGGGCgaauuuuuagaaCGCGCGUaCGCCUa -3' miRNA: 3'- -GGAGUU-----UGGa-UCCG------------GCGCGCA-GCGGG- -5' |
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31390 | 3' | -57.4 | NC_006623.1 | + | 145852 | 0.75 | 0.38727 |
Target: 5'- cCCUCccgacgagaaAGACCcGGGCCGCaGCGUgGCCa -3' miRNA: 3'- -GGAG----------UUUGGaUCCGGCG-CGCAgCGGg -5' |
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31390 | 3' | -57.4 | NC_006623.1 | + | 145934 | 0.66 | 0.888625 |
Target: 5'- uCCgcagaGGGCCgcgUAGGCguaCGCGCGuucuaaaaauUCGCCCg -3' miRNA: 3'- -GGag---UUUGG---AUCCG---GCGCGC----------AGCGGG- -5' |
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31390 | 3' | -57.4 | NC_006623.1 | + | 148212 | 0.7 | 0.670449 |
Target: 5'- cCCUgGAgGCC--GGCCGCGCGcaUGCCCc -3' miRNA: 3'- -GGAgUU-UGGauCCGGCGCGCa-GCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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