Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31390 | 3' | -57.4 | NC_006623.1 | + | 115887 | 0.67 | 0.836909 |
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Target: 5'- gUCUCAGaacguGCCccGGGCgaauuuuuagaaCGCGCGUaCGCCUa -3' miRNA: 3'- -GGAGUU-----UGGa-UCCG------------GCGCGCA-GCGGG- -5' |
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| 31390 | 3' | -57.4 | NC_006623.1 | + | 40542 | 0.67 | 0.836909 |
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Target: 5'- --cCAAGCCgcAGGUCGCcgcacgGCGUCGCUg -3' miRNA: 3'- ggaGUUUGGa-UCCGGCG------CGCAGCGGg -5' |
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| 31390 | 3' | -57.4 | NC_006623.1 | + | 4759 | 0.66 | 0.852672 |
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Target: 5'- cCCUaaaAAGgCUAGGCCGgucaGUggaaGUCGCCUa -3' miRNA: 3'- -GGAg--UUUgGAUCCGGCg---CG----CAGCGGG- -5' |
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| 31390 | 3' | -57.4 | NC_006623.1 | + | 123536 | 0.66 | 0.870571 |
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Target: 5'- cCCUCccggaAGACCUccccgaAGGuucccaaaacucgauCCGCGCGUUcgugGCCCa -3' miRNA: 3'- -GGAG-----UUUGGA------UCC---------------GGCGCGCAG----CGGG- -5' |
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| 31390 | 3' | -57.4 | NC_006623.1 | + | 145934 | 0.66 | 0.888625 |
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Target: 5'- uCCgcagaGGGCCgcgUAGGCguaCGCGCGuucuaaaaauUCGCCCg -3' miRNA: 3'- -GGag---UUUGG---AUCCG---GCGCGC----------AGCGGG- -5' |
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| 31390 | 3' | -57.4 | NC_006623.1 | + | 122450 | 1.14 | 0.00115 |
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Target: 5'- gCCUCAAACCUAGGCCGCGCGUCGCCCg -3' miRNA: 3'- -GGAGUUUGGAUCCGGCGCGCAGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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