Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31663 | 5' | -50.9 | NC_006883.1 | + | 41670 | 0.66 | 0.998844 |
Target: 5'- aCACCAGCAGGGuu-UUcCUUCCAg-- -3' miRNA: 3'- -GUGGUCGUCCUgguAAuGAAGGUaga -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 23253 | 0.66 | 0.997999 |
Target: 5'- uCACCAGCAGGACCAg----------- -3' miRNA: 3'- -GUGGUCGUCCUGGUaaugaagguaga -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 26971 | 0.66 | 0.997999 |
Target: 5'- aACCAGCAGGACCuugugaaACUgauacCCA-CUg -3' miRNA: 3'- gUGGUCGUCCUGGuaa----UGAa----GGUaGA- -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 232559 | 0.66 | 0.997999 |
Target: 5'- gCACCAGUAGuaGACCAcugUGCU-CUGUCa -3' miRNA: 3'- -GUGGUCGUC--CUGGUa--AUGAaGGUAGa -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 103363 | 0.66 | 0.997622 |
Target: 5'- aCACCAGCAauGGAUCcugGUUGaugaUCCAUCa -3' miRNA: 3'- -GUGGUCGU--CCUGG---UAAUga--AGGUAGa -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 23324 | 0.66 | 0.997187 |
Target: 5'- aACCAGCAGGACC-UUGaggaCCAg-- -3' miRNA: 3'- gUGGUCGUCCUGGuAAUgaa-GGUaga -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 222987 | 0.67 | 0.996118 |
Target: 5'- aACCAGCAGGACCAggugaaccUugUggaCCAg-- -3' miRNA: 3'- gUGGUCGUCCUGGUa-------AugAa--GGUaga -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 104241 | 0.67 | 0.993911 |
Target: 5'- gAUCuuGCAGGAgCAUUACUUCCu--- -3' miRNA: 3'- gUGGu-CGUCCUgGUAAUGAAGGuaga -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 38302 | 0.68 | 0.992983 |
Target: 5'- aACCaaGGCAGGACCAU-AUUUCgGUUc -3' miRNA: 3'- gUGG--UCGUCCUGGUAaUGAAGgUAGa -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 26786 | 0.68 | 0.992983 |
Target: 5'- gACCAGCAGGACCAguuggACcUgCAg-- -3' miRNA: 3'- gUGGUCGUCCUGGUaa---UGaAgGUaga -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 31855 | 0.68 | 0.992983 |
Target: 5'- cCACCAGCAGGACa---GCUUUUAg-- -3' miRNA: 3'- -GUGGUCGUCCUGguaaUGAAGGUaga -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 27233 | 0.68 | 0.992983 |
Target: 5'- -uCCAGCAGGuCC--UACaggUCCAUCa -3' miRNA: 3'- guGGUCGUCCuGGuaAUGa--AGGUAGa -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 194376 | 0.68 | 0.990791 |
Target: 5'- cCACCAGCAGcaccaccaACCAUUGCUccaCCAUUc -3' miRNA: 3'- -GUGGUCGUCc-------UGGUAAUGAa--GGUAGa -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 23151 | 0.68 | 0.988093 |
Target: 5'- gCACCAGCAGGACCAguuggaCCu--- -3' miRNA: 3'- -GUGGUCGUCCUGGUaaugaaGGuaga -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 223081 | 0.69 | 0.986533 |
Target: 5'- cCACCAGCAGGACCAggaggaCCu--- -3' miRNA: 3'- -GUGGUCGUCCUGGUaaugaaGGuaga -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 86791 | 0.69 | 0.98482 |
Target: 5'- aCAUCAGCAGaAUCAUUGauagUUCCAUCa -3' miRNA: 3'- -GUGGUCGUCcUGGUAAUg---AAGGUAGa -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 28467 | 0.7 | 0.973675 |
Target: 5'- cCACCAGUAGGACCuggaggUccuggaggaccACUuccUCCAUCa -3' miRNA: 3'- -GUGGUCGUCCUGGua----A-----------UGA---AGGUAGa -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 21968 | 0.7 | 0.970875 |
Target: 5'- gACCuGCAGGACCAggAgaUCCAg-- -3' miRNA: 3'- gUGGuCGUCCUGGUaaUgaAGGUaga -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 117560 | 0.72 | 0.940527 |
Target: 5'- cCACCAGCAuuuGGACCAgaaccuuuaaUUGCUUCCc--- -3' miRNA: 3'- -GUGGUCGU---CCUGGU----------AAUGAAGGuaga -5' |
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31663 | 5' | -50.9 | NC_006883.1 | + | 21726 | 0.72 | 0.940527 |
Target: 5'- gACCAGCAGGACCAguaggACcaccaccguUUCCA-CUa -3' miRNA: 3'- gUGGUCGUCCUGGUaa---UG---------AAGGUaGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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