Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31668 | 5' | -61.1 | NC_006883.1 | + | 23350 | 0.66 | 0.793817 |
Target: 5'- aCCuaCaGGACCaGuuGGACCUGGAGa -3' miRNA: 3'- aGGcgGaCCUGGaCcuCCUGGACCUC- -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 23251 | 0.67 | 0.767857 |
Target: 5'- aCCaGCa-GGACCaGuuGGACCUGGAGg -3' miRNA: 3'- aGG-CGgaCCUGGaCcuCCUGGACCUC- -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 21902 | 0.67 | 0.740946 |
Target: 5'- gCgGaCCUugcGGACCUGGAGGACCaccagcaGGAc -3' miRNA: 3'- aGgC-GGA---CCUGGACCUCCUGGa------CCUc -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 26550 | 0.67 | 0.7318 |
Target: 5'- cCUG-CUGGACCUGuAGGACCugcUGGAc -3' miRNA: 3'- aGGCgGACCUGGACcUCCUGG---ACCUc -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 21715 | 0.68 | 0.713287 |
Target: 5'- gUCCuCCUGGuCCaagUGGAGGuCCUGGu- -3' miRNA: 3'- -AGGcGGACCuGG---ACCUCCuGGACCuc -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 23293 | 0.68 | 0.685084 |
Target: 5'- aCCGauaCCUggaGGACCUuGAGGACCaGGAGc -3' miRNA: 3'- aGGC---GGA---CCUGGAcCUCCUGGaCCUC- -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 22030 | 0.69 | 0.666078 |
Target: 5'- aCCGUCUGGACCcGuAGGACCagcaGGAc -3' miRNA: 3'- aGGCGGACCUGGaCcUCCUGGa---CCUc -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 28578 | 0.69 | 0.666078 |
Target: 5'- aCUGuagcaCCUGaaguuCCUGGuGGACCUGGAGg -3' miRNA: 3'- aGGC-----GGACcu---GGACCuCCUGGACCUC- -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 27005 | 0.69 | 0.646975 |
Target: 5'- aCUGUUUGaaaaccagaACCaGGAGGACCUGGAGa -3' miRNA: 3'- aGGCGGACc--------UGGaCCUCCUGGACCUC- -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 23402 | 0.69 | 0.627831 |
Target: 5'- gUCCagUUGGACCUGuGGGACCUGuGGGa -3' miRNA: 3'- -AGGcgGACCUGGACcUCCUGGAC-CUC- -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 22132 | 0.69 | 0.622088 |
Target: 5'- aCCaCUUGGACCagcaGGAccaucuccaccacucGGACCUGGAGg -3' miRNA: 3'- aGGcGGACCUGGa---CCU---------------CCUGGACCUC- -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 28375 | 0.7 | 0.599153 |
Target: 5'- aCCGUCaGcACCaGGAGGuCCUGGAGg -3' miRNA: 3'- aGGCGGaCcUGGaCCUCCuGGACCUC- -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 26728 | 0.7 | 0.581078 |
Target: 5'- aUCUGCUccaucauuuccaggaGGACCUGcuGGACCUGGAGu -3' miRNA: 3'- -AGGCGGa--------------CCUGGACcuCCUGGACCUC- -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 27594 | 0.7 | 0.570664 |
Target: 5'- uUCCaggaGGACCUGGAGGACCugcUGGAc -3' miRNA: 3'- -AGGcggaCCUGGACCUCCUGG---ACCUc -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 23698 | 0.71 | 0.551852 |
Target: 5'- aCCagaUGGACCUGGAGGACCagcaGGAc -3' miRNA: 3'- aGGcggACCUGGACCUCCUGGa---CCUc -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 28102 | 0.71 | 0.533233 |
Target: 5'- aCCagUUGGACCUGGAGGACCagcaGGAc -3' miRNA: 3'- aGGcgGACCUGGACCUCCUGGa---CCUc -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 27666 | 0.71 | 0.524007 |
Target: 5'- uUCCaggaGGACCUGGAGGACCUgcuggaccuGGAGu -3' miRNA: 3'- -AGGcggaCCUGGACCUCCUGGA---------CCUC- -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 23140 | 0.71 | 0.514842 |
Target: 5'- aCCagUUGGACCUGGAGcACCUGGAu -3' miRNA: 3'- aGGcgGACCUGGACCUCcUGGACCUc -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 27493 | 0.72 | 0.487759 |
Target: 5'- cCUG-CUGGACCUGuAGGACCaGGAGc -3' miRNA: 3'- aGGCgGACCUGGACcUCCUGGaCCUC- -5' |
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31668 | 5' | -61.1 | NC_006883.1 | + | 21744 | 0.72 | 0.444195 |
Target: 5'- uUCCagUUGGACCUGGAGGACCagcaGGAc -3' miRNA: 3'- -AGGcgGACCUGGACCUCCUGGa---CCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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