Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31720 | 5' | -59.1 | NC_006938.1 | + | 60038 | 0.66 | 0.578153 |
Target: 5'- cCCGGCcuugccgCCGUgGUaCCAgGCCG-CGUCg -3' miRNA: 3'- -GGCCGa------GGCAgCG-GGUgUGGCaGUAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 4988 | 0.66 | 0.567773 |
Target: 5'- cCCGGCcaUCCacgagCGCCC-CACCGaugcgCGUCc -3' miRNA: 3'- -GGCCG--AGGca---GCGGGuGUGGCa----GUAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 3401 | 0.66 | 0.561568 |
Target: 5'- aCCGGCUCCGgCGacaauggucgucugcCCCGCGCCcaccuGUCuagCg -3' miRNA: 3'- -GGCCGAGGCaGC---------------GGGUGUGG-----CAGua-G- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 58794 | 0.66 | 0.557441 |
Target: 5'- cUCGGCgaCUGaacCGCgUACACCGUCAUUc -3' miRNA: 3'- -GGCCGa-GGCa--GCGgGUGUGGCAGUAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 15960 | 0.67 | 0.547163 |
Target: 5'- -aGGC-CCGaUCGCCgACGCgGUCGa- -3' miRNA: 3'- ggCCGaGGC-AGCGGgUGUGgCAGUag -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 43145 | 0.67 | 0.547163 |
Target: 5'- aUCGGCUCCGUgGCUCcgacagACACgGugcgguucuUCGUCa -3' miRNA: 3'- -GGCCGAGGCAgCGGG------UGUGgC---------AGUAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 57442 | 0.67 | 0.546138 |
Target: 5'- gUGGCUCCGgcauagUCGCCCcaugccucggccaGgGCCGUC-UCu -3' miRNA: 3'- gGCCGAGGC------AGCGGG-------------UgUGGCAGuAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 44742 | 0.67 | 0.526795 |
Target: 5'- gCCGcGCgcccUCGUCGCCCGCgACCacCAUCu -3' miRNA: 3'- -GGC-CGa---GGCAGCGGGUG-UGGcaGUAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 41148 | 0.67 | 0.526795 |
Target: 5'- gCUGGCUCCuUCGCCgCGCgggACCGa-GUCa -3' miRNA: 3'- -GGCCGAGGcAGCGG-GUG---UGGCagUAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 55458 | 0.67 | 0.526795 |
Target: 5'- aUCGGgUCCGUCGUCggCACACUGcUCuUCc -3' miRNA: 3'- -GGCCgAGGCAGCGG--GUGUGGC-AGuAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 55258 | 0.67 | 0.526795 |
Target: 5'- aUCGGCagUCUGUcauucaCGCCCACGCCacccggcgcaagGUCAUUc -3' miRNA: 3'- -GGCCG--AGGCA------GCGGGUGUGG------------CAGUAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 43810 | 0.67 | 0.516718 |
Target: 5'- cCCGGUugUCCaG-CGCCUuccGCACCG-CGUCg -3' miRNA: 3'- -GGCCG--AGG-CaGCGGG---UGUGGCaGUAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 53507 | 0.67 | 0.513709 |
Target: 5'- aCCGGCUCgaugccagaggacaCGUUGUCC--GCCGUCAg- -3' miRNA: 3'- -GGCCGAG--------------GCAGCGGGugUGGCAGUag -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 38141 | 0.68 | 0.458066 |
Target: 5'- aCCGGCU-CGUCGCCgAUguaGCCGUgGc- -3' miRNA: 3'- -GGCCGAgGCAGCGGgUG---UGGCAgUag -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 39875 | 0.68 | 0.439312 |
Target: 5'- cUCGGCcaUGcCGCCCugGCCGaCGUCg -3' miRNA: 3'- -GGCCGagGCaGCGGGugUGGCaGUAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 48413 | 0.69 | 0.412024 |
Target: 5'- aCCGGCUCCGgagcgacgacCGCCC-CuCCGUUccggGUCu -3' miRNA: 3'- -GGCCGAGGCa---------GCGGGuGuGGCAG----UAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 45162 | 0.69 | 0.403163 |
Target: 5'- gCgGGCUCCcuuggUGCCCuuGCCGUUGUCc -3' miRNA: 3'- -GgCCGAGGca---GCGGGugUGGCAGUAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 37866 | 0.7 | 0.369787 |
Target: 5'- uCUGGCUCCaGgagauccaggagaagCGCCCACACUGguggcccgCGUCu -3' miRNA: 3'- -GGCCGAGG-Ca--------------GCGGGUGUGGCa-------GUAG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 41847 | 0.7 | 0.368956 |
Target: 5'- aCCGGCUCUGUCggGCUgGaguuGCCGUCGcUCa -3' miRNA: 3'- -GGCCGAGGCAG--CGGgUg---UGGCAGU-AG- -5' |
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31720 | 5' | -59.1 | NC_006938.1 | + | 55897 | 0.7 | 0.352614 |
Target: 5'- -gGGCUCCGgccaggUGCCCggACACCGUggccucugcuaCAUCg -3' miRNA: 3'- ggCCGAGGCa-----GCGGG--UGUGGCA-----------GUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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