Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31747 | 5' | -59.5 | NC_006938.1 | + | 47051 | 0.66 | 0.510783 |
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Target: 5'- -aUCGUGCGAcagACGUCCAuGGCGGuGCg -3' miRNA: 3'- cgGGCGCGCU---UGCGGGU-CCGUCuUGa -5' |
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| 31747 | 5' | -59.5 | NC_006938.1 | + | 50017 | 0.66 | 0.541399 |
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Target: 5'- cGCCCGUGUGggUGgCCccGCcucuGGAACg -3' miRNA: 3'- -CGGGCGCGCuuGCgGGucCG----UCUUGa -5' |
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| 31747 | 5' | -59.5 | NC_006938.1 | + | 22380 | 0.66 | 0.545529 |
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Target: 5'- gGgCCGCGCGAcuggugcagaaccccGCGaCCCAGaGCGaGACg -3' miRNA: 3'- -CgGGCGCGCU---------------UGC-GGGUC-CGUcUUGa -5' |
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| 31747 | 5' | -59.5 | NC_006938.1 | + | 20439 | 0.66 | 0.548633 |
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Target: 5'- uGCCuggCGgGCGGACGCugCCAGGUgcugaucaaggugaAGAACg -3' miRNA: 3'- -CGG---GCgCGCUUGCG--GGUCCG--------------UCUUGa -5' |
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| 31747 | 5' | -59.5 | NC_006938.1 | + | 13513 | 0.66 | 0.551743 |
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Target: 5'- aCCCGCGcCGGGCacaaagGCCUcucgcguuccagAGGCGGGGCc -3' miRNA: 3'- cGGGCGC-GCUUG------CGGG------------UCCGUCUUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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