Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31747 | 5' | -59.5 | NC_006938.1 | + | 58582 | 0.67 | 0.490778 |
|
Target: 5'- cGCUCGUG-GAugGCCUGGGCAcugucccccuGGGCg -3' miRNA: 3'- -CGGGCGCgCUugCGGGUCCGU----------CUUGa -5' |
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| 31747 | 5' | -59.5 | NC_006938.1 | + | 59089 | 0.71 | 0.279727 |
|
Target: 5'- cUUCGUGguugucaacaCGAGCGCCCAGGCAGuuACUg -3' miRNA: 3'- cGGGCGC----------GCUUGCGGGUCCGUCu-UGA- -5' |
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| 31747 | 5' | -59.5 | NC_006938.1 | + | 59230 | 0.67 | 0.451917 |
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Target: 5'- aGCUCG-GCGAGCGCggucuCCAGGCGcGggUUc -3' miRNA: 3'- -CGGGCgCGCUUGCG-----GGUCCGU-CuuGA- -5' |
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| 31747 | 5' | -59.5 | NC_006938.1 | + | 60857 | 0.66 | 0.510783 |
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Target: 5'- aCCCuuGUuuacacGAGCGCCC-GGCGGGACa -3' miRNA: 3'- cGGGcgCG------CUUGCGGGuCCGUCUUGa -5' |
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| 31747 | 5' | -59.5 | NC_006938.1 | + | 63470 | 0.68 | 0.433127 |
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Target: 5'- cGCUCGCGagcuGAACGUCCccaucgagugGGGCGGuGACUg -3' miRNA: 3'- -CGGGCGCg---CUUGCGGG----------UCCGUC-UUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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