Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 31790 | 5' | -56.3 | NC_006938.1 | + | 51266 | 0.66 | 0.666526 |
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Target: 5'- uGGCGGCGAUGgCA--GCCGUCagcgcgCGGa -3' miRNA: 3'- -CCGUCGUUACgGUucCGGCAGga----GCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 39543 | 0.66 | 0.6546 |
|
Target: 5'- cGGUGGCAcgGCCAccGcucgcacggucucGGCCGUCgacuuCUCGGa -3' miRNA: 3'- -CCGUCGUuaCGGU--U-------------CCGGCAG-----GAGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 47010 | 0.66 | 0.666526 |
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Target: 5'- gGGUGGCAAcgacgcUGCCcugggcGGCCGaaUCUUCGAg -3' miRNA: 3'- -CCGUCGUU------ACGGuu----CCGGC--AGGAGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 48352 | 0.66 | 0.666526 |
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Target: 5'- gGGCGGCGGUGaCGAGcGC-GUCCugUCGGa -3' miRNA: 3'- -CCGUCGUUACgGUUC-CGgCAGG--AGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 13981 | 0.66 | 0.666526 |
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Target: 5'- uGGCgcuGGCgAAUGUagaacuCGAGGUCGUCgUCGAg -3' miRNA: 3'- -CCG---UCG-UUACG------GUUCCGGCAGgAGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 4278 | 0.66 | 0.655685 |
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Target: 5'- cGGCGGUcagcagggucucGAUGUCGGaGCCGUCCgccCGGa -3' miRNA: 3'- -CCGUCG------------UUACGGUUcCGGCAGGa--GCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 6991 | 0.66 | 0.655685 |
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Target: 5'- cGGCuggggaGGCAagGCCGGGGCCG-CUcUGAu -3' miRNA: 3'- -CCG------UCGUuaCGGUUCCGGCaGGaGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 45793 | 0.66 | 0.677334 |
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Target: 5'- cGGCAaCAagGCCGAGGCCcgugccacGUUCUCc- -3' miRNA: 3'- -CCGUcGUuaCGGUUCCGG--------CAGGAGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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