Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31790 | 5' | -56.3 | NC_006938.1 | + | 48654 | 0.68 | 0.547662 |
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Target: 5'- uGGaGGCGA--CCAAGGCCGUCCg-GAa -3' miRNA: 3'- -CCgUCGUUacGGUUCCGGCAGGagCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 51266 | 0.66 | 0.666526 |
|
Target: 5'- uGGCGGCGAUGgCA--GCCGUCagcgcgCGGa -3' miRNA: 3'- -CCGUCGUUACgGUucCGGCAGga----GCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 51497 | 0.67 | 0.633951 |
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Target: 5'- uGGCAGCGGcgGCgAuGGCCGgccacacgCuCUCGAa -3' miRNA: 3'- -CCGUCGUUa-CGgUuCCGGCa-------G-GAGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 52027 | 0.66 | 0.677334 |
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Target: 5'- gGGCGGCAccGCCGAGcccaccGCCG-CCU-GAc -3' miRNA: 3'- -CCGUCGUuaCGGUUC------CGGCaGGAgCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 54115 | 0.71 | 0.399012 |
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Target: 5'- cGGCAGCuuUGCCAAc-CUGUCCUCu- -3' miRNA: 3'- -CCGUCGuuACGGUUccGGCAGGAGcu -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 54409 | 0.68 | 0.558302 |
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Target: 5'- cGCGGCcggaGUCGAGGCCaccacuGUCCUCGc -3' miRNA: 3'- cCGUCGuua-CGGUUCCGG------CAGGAGCu -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 61013 | 0.68 | 0.568999 |
|
Target: 5'- aGGaacucuGgAGUGCUGAGGUccgCGUCCUCGAa -3' miRNA: 3'- -CCgu----CgUUACGGUUCCG---GCAGGAGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 63611 | 0.78 | 0.140604 |
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Target: 5'- gGGCAGC--UGCCAGugcGGuCCGUCCUUGAa -3' miRNA: 3'- -CCGUCGuuACGGUU---CC-GGCAGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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