Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31790 | 5' | -56.3 | NC_006938.1 | + | 52027 | 0.66 | 0.677334 |
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Target: 5'- gGGCGGCAccGCCGAGcccaccGCCG-CCU-GAc -3' miRNA: 3'- -CCGUCGUuaCGGUUC------CGGCaGGAgCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 18594 | 0.66 | 0.677334 |
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Target: 5'- uGCGGC-AUGCCc-GGCgCGUCCgaggCGGc -3' miRNA: 3'- cCGUCGuUACGGuuCCG-GCAGGa---GCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 45966 | 0.66 | 0.677334 |
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Target: 5'- cGuCAGCGAggaCAGGGCCGUCCg--- -3' miRNA: 3'- cC-GUCGUUacgGUUCCGGCAGGagcu -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 22505 | 0.66 | 0.677334 |
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Target: 5'- uGCcGCGcAUGaCCAGGGCCGU-CUCGc -3' miRNA: 3'- cCGuCGU-UAC-GGUUCCGGCAgGAGCu -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 22027 | 0.66 | 0.688101 |
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Target: 5'- cGGCGGCAAgaUCAAGGUCGgCUaCGAg -3' miRNA: 3'- -CCGUCGUUacGGUUCCGGCaGGaGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 44954 | 0.66 | 0.688101 |
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Target: 5'- cGGgAGCcGUGCCAAGGUggacuguuccacUGUCUgCGAu -3' miRNA: 3'- -CCgUCGuUACGGUUCCG------------GCAGGaGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 41573 | 0.66 | 0.698816 |
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Target: 5'- cGCGGCg--GCCuGGGCaaaGUCCcCGAc -3' miRNA: 3'- cCGUCGuuaCGGuUCCGg--CAGGaGCU- -5' |
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| 31790 | 5' | -56.3 | NC_006938.1 | + | 31851 | 0.66 | 0.698816 |
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Target: 5'- cGUcGCGcgGUCGAGGgUGUCCUgGAg -3' miRNA: 3'- cCGuCGUuaCGGUUCCgGCAGGAgCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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