Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31898 | 3' | -62.1 | NC_006938.1 | + | 15653 | 0.7 | 0.246103 |
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Target: 5'- aG-CGCGGUCGGcGUCGAGGUCGg-- -3' miRNA: 3'- aCaGCGCCGGCCuCAGCUCCGGUggu -5' |
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| 31898 | 3' | -62.1 | NC_006938.1 | + | 26880 | 0.7 | 0.240138 |
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Target: 5'- -uUCGCGGUCGGGGcuaCGAGGacaACCAg -3' miRNA: 3'- acAGCGCCGGCCUCa--GCUCCgg-UGGU- -5' |
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| 31898 | 3' | -62.1 | NC_006938.1 | + | 28671 | 0.7 | 0.234294 |
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Target: 5'- cGUCGCGGCUgGGAGU-GGGGCUcguuCCu -3' miRNA: 3'- aCAGCGCCGG-CCUCAgCUCCGGu---GGu -5' |
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| 31898 | 3' | -62.1 | NC_006938.1 | + | 15798 | 0.71 | 0.201701 |
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Target: 5'- -cUC-CGGCCGGAGgaGAGGCCuCCAg -3' miRNA: 3'- acAGcGCCGGCCUCagCUCCGGuGGU- -5' |
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| 31898 | 3' | -62.1 | NC_006938.1 | + | 38932 | 0.71 | 0.182223 |
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Target: 5'- aUGcCGUGGCCGGGGUCGuAGcGCCGg-- -3' miRNA: 3'- -ACaGCGCCGGCCUCAGC-UC-CGGUggu -5' |
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| 31898 | 3' | -62.1 | NC_006938.1 | + | 42423 | 0.74 | 0.12999 |
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Target: 5'- cGaCGCGGCCGGAG-CGAcGCCuGCCAc -3' miRNA: 3'- aCaGCGCCGGCCUCaGCUcCGG-UGGU- -5' |
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| 31898 | 3' | -62.1 | NC_006938.1 | + | 13585 | 0.79 | 0.049038 |
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Target: 5'- aG-CGCGGCCGGAGUCGAcaggaguggaGGUCACUAc -3' miRNA: 3'- aCaGCGCCGGCCUCAGCU----------CCGGUGGU- -5' |
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| 31898 | 3' | -62.1 | NC_006938.1 | + | 51475 | 0.84 | 0.023805 |
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Target: 5'- cGUCGCGGCCGGAgccggggcaGUCGAGGCgAUCGu -3' miRNA: 3'- aCAGCGCCGGCCU---------CAGCUCCGgUGGU- -5' |
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| 31898 | 3' | -62.1 | NC_006938.1 | + | 54405 | 1.07 | 0.000376 |
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Target: 5'- cUGUCGCGGCCGGAGUCGAGGCCACCAc -3' miRNA: 3'- -ACAGCGCCGGCCUCAGCUCCGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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