Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32070 | 5' | -58.7 | NC_007016.1 | + | 20251 | 0.66 | 0.79449 |
Target: 5'- cGGGGUCcCGuuuaacauugccAGCUACGCCC--UCCUg -3' miRNA: 3'- aCCCCAGaGU------------UUGGUGCGGGgcAGGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 116078 | 0.66 | 0.79449 |
Target: 5'- cGGcGGUCaUCAGaacauAUCACcCCCCGcgCCCg -3' miRNA: 3'- aCC-CCAG-AGUU-----UGGUGcGGGGCa-GGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 123131 | 0.66 | 0.790024 |
Target: 5'- aGGGGUUUCucugaguAGACCgaGCGCCCCcaggagcuggaaCCCa -3' miRNA: 3'- aCCCCAGAG-------UUUGG--UGCGGGGca----------GGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 17099 | 0.66 | 0.785523 |
Target: 5'- cUGGGccaGUC-CGGACC-CGUCCUG-CCCa -3' miRNA: 3'- -ACCC---CAGaGUUUGGuGCGGGGCaGGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 130339 | 0.66 | 0.785523 |
Target: 5'- aGGGGggCUgGGGCCcgggGCGCCUCGgcggggccggCCCc -3' miRNA: 3'- aCCCCa-GAgUUUGG----UGCGGGGCa---------GGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 114667 | 0.66 | 0.780078 |
Target: 5'- -uGGGUauugcgCAAugccaagcucccgcuACCAUGCCgCGUCCCg -3' miRNA: 3'- acCCCAga----GUU---------------UGGUGCGGgGCAGGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 130170 | 0.66 | 0.773668 |
Target: 5'- --cGGUC-CGGccgagcccggcggcGCCGCGCCCCGacgCCCc -3' miRNA: 3'- accCCAGaGUU--------------UGGUGCGGGGCa--GGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 14891 | 0.66 | 0.757856 |
Target: 5'- gGGGGUUaUCAGGgCGCGacggugauuaaCCCCaUCCCg -3' miRNA: 3'- aCCCCAG-AGUUUgGUGC-----------GGGGcAGGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 129947 | 0.67 | 0.748407 |
Target: 5'- cGaGGGUCUC---CCGCGCggccCCCGggCCCc -3' miRNA: 3'- aC-CCCAGAGuuuGGUGCG----GGGCa-GGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 123887 | 0.67 | 0.748407 |
Target: 5'- aGGGccaUCUCAAuagcggugaccACUACGCCgCCGUCgCu -3' miRNA: 3'- aCCCc--AGAGUU-----------UGGUGCGG-GGCAGgG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 72104 | 0.67 | 0.738859 |
Target: 5'- cGGGGagaagucaacgUCUCAcucaucgcguuuAugUACGCCUCG-CCCg -3' miRNA: 3'- aCCCC-----------AGAGU------------UugGUGCGGGGCaGGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 50196 | 0.67 | 0.729221 |
Target: 5'- gGGGGUCUCgAAACUgaACuGuCCCCG-CCg -3' miRNA: 3'- aCCCCAGAG-UUUGG--UG-C-GGGGCaGGg -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 78390 | 0.67 | 0.729221 |
Target: 5'- -cGGGUaaCGcAACCGCGCCCCcuucacucucGUCCUg -3' miRNA: 3'- acCCCAgaGU-UUGGUGCGGGG----------CAGGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 87857 | 0.67 | 0.718526 |
Target: 5'- gGGGGUCgc---CCugGCuCCCGUaaaagacCCCa -3' miRNA: 3'- aCCCCAGaguuuGGugCG-GGGCA-------GGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 115589 | 0.67 | 0.716572 |
Target: 5'- cGGGG-CUCGAcggcuguggcACCGCGCCagcgauguuuacggCCGuaaUCCCu -3' miRNA: 3'- aCCCCaGAGUU----------UGGUGCGG--------------GGC---AGGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 42613 | 0.67 | 0.709711 |
Target: 5'- -uGGGUCgu-AACUACGCCCUGgacauggacCCCg -3' miRNA: 3'- acCCCAGaguUUGGUGCGGGGCa--------GGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 121335 | 0.67 | 0.699855 |
Target: 5'- cGGGGUgUCAccaggaAACCGCGUCUC--CCCu -3' miRNA: 3'- aCCCCAgAGU------UUGGUGCGGGGcaGGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 16831 | 0.67 | 0.699855 |
Target: 5'- aGGcGUCUCAGACguCGCCCCacucuuucGUCgCg -3' miRNA: 3'- aCCcCAGAGUUUGguGCGGGG--------CAGgG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 15919 | 0.67 | 0.699855 |
Target: 5'- cGGcGGUCgcuugCAcaacAGCCGCGCCgCCG-CCUu -3' miRNA: 3'- aCC-CCAGa----GU----UUGGUGCGG-GGCaGGG- -5' |
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32070 | 5' | -58.7 | NC_007016.1 | + | 18880 | 0.67 | 0.696888 |
Target: 5'- cGGGGUCgcgcguuucgCAAACaCACGagguuucccagacaCCCCcUCCCg -3' miRNA: 3'- aCCCCAGa---------GUUUG-GUGC--------------GGGGcAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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