Results 1 - 20 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32077 | 5' | -61.3 | NC_007016.1 | + | 17260 | 0.66 | 0.674791 |
Target: 5'- gGGUgggCAGGaCGGGU--CCGGAcUGGCCc -3' miRNA: 3'- -CCGa--GUCC-GUCCGguGGCCUaGCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 130278 | 0.66 | 0.674791 |
Target: 5'- gGGCccggCGGaGCGGGaCC-CgGGggCGGCCc -3' miRNA: 3'- -CCGa---GUC-CGUCC-GGuGgCCuaGCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 124928 | 0.66 | 0.674791 |
Target: 5'- cGGCcgAGGUcGGCUGUCuGAUCGGCCa -3' miRNA: 3'- -CCGagUCCGuCCGGUGGcCUAGCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 15263 | 0.66 | 0.674791 |
Target: 5'- cGGUUUA--CGGGuUCACCGGggUGGCCa -3' miRNA: 3'- -CCGAGUccGUCC-GGUGGCCuaGCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 13259 | 0.66 | 0.673804 |
Target: 5'- cGGCUCuGGCGGGCgGCaaguacaggcguaUGGGUgUGGUUa -3' miRNA: 3'- -CCGAGuCCGUCCGgUG-------------GCCUA-GCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 68055 | 0.66 | 0.66392 |
Target: 5'- uGCgauaugCGGGCcggGGGCCG-CGGAUguugaacCGGCCa -3' miRNA: 3'- cCGa-----GUCCG---UCCGGUgGCCUA-------GCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 76881 | 0.66 | 0.655995 |
Target: 5'- aGGCUCuGGC-GGCCuuuauugaggaagguCUGGcAUgGGCCc -3' miRNA: 3'- -CCGAGuCCGuCCGGu--------------GGCC-UAgCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 72736 | 0.66 | 0.655003 |
Target: 5'- gGGCgggaguugagCGGGUGGGUUGCgGGAagcgUGGCCa -3' miRNA: 3'- -CCGa---------GUCCGUCCGGUGgCCUa---GCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 110025 | 0.66 | 0.655003 |
Target: 5'- gGGUgcaUUAGGCagGGGCC-CUGuGAUUGGUCa -3' miRNA: 3'- -CCG---AGUCCG--UCCGGuGGC-CUAGCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 16908 | 0.66 | 0.655003 |
Target: 5'- cGGUUaCAGGUgAGGCCGuuuUCGGAUgacgCGGCa -3' miRNA: 3'- -CCGA-GUCCG-UCCGGU---GGCCUA----GCCGg -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 130037 | 0.66 | 0.635146 |
Target: 5'- cGGC-CGGGCgccuggGGGCCcccGCCGGG-CGccGCCg -3' miRNA: 3'- -CCGaGUCCG------UCCGG---UGGCCUaGC--CGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 14769 | 0.66 | 0.635146 |
Target: 5'- uGGCcaaaauUCAGGcCAGGCgCGuccuaaCGGA-CGGCCa -3' miRNA: 3'- -CCG------AGUCC-GUCCG-GUg-----GCCUaGCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 116136 | 0.66 | 0.635146 |
Target: 5'- uGGC-CGGGCAGag-GCCGGc-CGGCCa -3' miRNA: 3'- -CCGaGUCCGUCcggUGGCCuaGCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 115813 | 0.66 | 0.625211 |
Target: 5'- gGGCUC-GGUGGGgCGCCGGc---GCCa -3' miRNA: 3'- -CCGAGuCCGUCCgGUGGCCuagcCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 5256 | 0.67 | 0.61528 |
Target: 5'- aGGCgUCAauacucGGCAacaGGCCAuCUGGAUCuGGCg -3' miRNA: 3'- -CCG-AGU------CCGU---CCGGU-GGCCUAG-CCGg -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 130403 | 0.67 | 0.595462 |
Target: 5'- uGC-CGGGCGgcGGCCGCCcGGcAUCGggaGCCg -3' miRNA: 3'- cCGaGUCCGU--CCGGUGG-CC-UAGC---CGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 37781 | 0.67 | 0.592497 |
Target: 5'- uGGC-CAGGCAGcaaaaaauuGCCAguugaguuuugaaaUCGGAaUUGGCCg -3' miRNA: 3'- -CCGaGUCCGUC---------CGGU--------------GGCCU-AGCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 115534 | 0.67 | 0.592497 |
Target: 5'- aGC-CGGGggauuaaGGGCCGCCGGcagggacguuucccAUUGGCCc -3' miRNA: 3'- cCGaGUCCg------UCCGGUGGCC--------------UAGCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 71308 | 0.67 | 0.585588 |
Target: 5'- aGGC-CAaaaucGC-GGCCGCCGuuUCGGCCu -3' miRNA: 3'- -CCGaGUc----CGuCCGGUGGCcuAGCCGG- -5' |
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32077 | 5' | -61.3 | NC_007016.1 | + | 129886 | 0.67 | 0.56594 |
Target: 5'- gGGCcccCAGGCGcccggccggcGGCgGCgCGGGgccgCGGCCg -3' miRNA: 3'- -CCGa--GUCCGU----------CCGgUG-GCCUa---GCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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