Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32275 | 3' | -43.9 | NC_007023.1 | + | 174834 | 0.66 | 1 |
Target: 5'- -gAAUCCAUCauuacCGCGAUCAGC-CGu -3' miRNA: 3'- gaUUAGGUAGgua--GUGUUAGUUGuGU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 141012 | 0.66 | 1 |
Target: 5'- -aGAUCgAUCC-UUACugguAUCAACGCGa -3' miRNA: 3'- gaUUAGgUAGGuAGUGu---UAGUUGUGU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 73945 | 0.66 | 1 |
Target: 5'- ---uUCCAUCCAguuCAAUCAGgAUAa -3' miRNA: 3'- gauuAGGUAGGUaguGUUAGUUgUGU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 41037 | 0.66 | 1 |
Target: 5'- ---cUCCAUCCAUUugaaagauuAUAAUCAuCACGg -3' miRNA: 3'- gauuAGGUAGGUAG---------UGUUAGUuGUGU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 20930 | 0.66 | 1 |
Target: 5'- --cGUCCAUCgacaCAUCAagaaGGUUAGCACGa -3' miRNA: 3'- gauUAGGUAG----GUAGUg---UUAGUUGUGU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 65687 | 0.66 | 1 |
Target: 5'- uUAAUCaGUUCAcCGCAAUCAGgACAc -3' miRNA: 3'- gAUUAGgUAGGUaGUGUUAGUUgUGU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 166048 | 0.67 | 0.999999 |
Target: 5'- --cGUUCAcgCCGUCGC--UCAACACAg -3' miRNA: 3'- gauUAGGUa-GGUAGUGuuAGUUGUGU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 49261 | 0.67 | 0.999999 |
Target: 5'- ----cCCAUUCAUUACAAUaaCAGCGCc -3' miRNA: 3'- gauuaGGUAGGUAGUGUUA--GUUGUGu -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 146061 | 0.67 | 0.999999 |
Target: 5'- gUAAUcCCAUUUuggAUCACAGUCugGACGCAu -3' miRNA: 3'- gAUUA-GGUAGG---UAGUGUUAG--UUGUGU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 54297 | 0.67 | 0.999999 |
Target: 5'- cCUGAUCCGauaUCCAuuauaagaaccauuUCGCAAaugCAGCGCc -3' miRNA: 3'- -GAUUAGGU---AGGU--------------AGUGUUa--GUUGUGu -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 92951 | 0.67 | 0.999999 |
Target: 5'- --uGUCCcUCCAUCGCu-UCAACGa- -3' miRNA: 3'- gauUAGGuAGGUAGUGuuAGUUGUgu -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 17619 | 0.68 | 0.999997 |
Target: 5'- -gGAUCUAUCCGUCgaagaaagGCGAUC-ACAUg -3' miRNA: 3'- gaUUAGGUAGGUAG--------UGUUAGuUGUGu -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 19112 | 0.68 | 0.999997 |
Target: 5'- -aGAUCCAUUCAUaCACAccaggcggcauGUCAACGgAa -3' miRNA: 3'- gaUUAGGUAGGUA-GUGU-----------UAGUUGUgU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 121996 | 0.68 | 0.999997 |
Target: 5'- -aGAUCCcugguaguAUUCGUUACGAcaUCAGCGCAa -3' miRNA: 3'- gaUUAGG--------UAGGUAGUGUU--AGUUGUGU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 123488 | 0.68 | 0.999992 |
Target: 5'- uUAGUgCAUCCGUCACu-UCGACu-- -3' miRNA: 3'- gAUUAgGUAGGUAGUGuuAGUUGugu -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 173716 | 0.68 | 0.999992 |
Target: 5'- -aGAUCCGUucCCGUCACAG-CAuacucGCACGu -3' miRNA: 3'- gaUUAGGUA--GGUAGUGUUaGU-----UGUGU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 79928 | 0.68 | 0.999992 |
Target: 5'- -gAGUCCAUCCAaaCGCuuUCAGC-CAg -3' miRNA: 3'- gaUUAGGUAGGUa-GUGuuAGUUGuGU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 58757 | 0.69 | 0.999988 |
Target: 5'- ---uUCCAaCCAUUACGAUCGccCACAa -3' miRNA: 3'- gauuAGGUaGGUAGUGUUAGUu-GUGU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 124802 | 0.69 | 0.999977 |
Target: 5'- -aAGUCCcgcuGUCCuUCAUAGUUAGCAUAu -3' miRNA: 3'- gaUUAGG----UAGGuAGUGUUAGUUGUGU- -5' |
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32275 | 3' | -43.9 | NC_007023.1 | + | 162879 | 0.69 | 0.999977 |
Target: 5'- aUAGUagCAUcaCCAUCGgAAUCAACACAu -3' miRNA: 3'- gAUUAg-GUA--GGUAGUgUUAGUUGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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