Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33030 | 5' | -56.5 | NC_007497.1 | + | 32656 | 0.71 | 0.344599 |
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Target: 5'- uUCGguuggCUCGGcAAUGCGAUCGGACuugGCGCg -3' miRNA: 3'- -AGUa----GGGCC-UUGCGCUGGCCUG---UGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 32832 | 0.69 | 0.42476 |
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Target: 5'- -aGUCcgCCGaGAACGCcGCUGGGCGCGCc -3' miRNA: 3'- agUAG--GGC-CUUGCGcUGGCCUGUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 34592 | 0.69 | 0.453849 |
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Target: 5'- ----gCCGuuuGCGCGACCGcGGCACGCu -3' miRNA: 3'- aguagGGCcu-UGCGCUGGC-CUGUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 34946 | 0.68 | 0.482966 |
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Target: 5'- -aAUCgCCGGGACgccguauGCGGCCGuGAuCGCGCg -3' miRNA: 3'- agUAG-GGCCUUG-------CGCUGGC-CU-GUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 38140 | 0.8 | 0.085384 |
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Target: 5'- cUCGUCCCGGAucacguucgcGCcgGCGACCGuGGCGCGCc -3' miRNA: 3'- -AGUAGGGCCU----------UG--CGCUGGC-CUGUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 38589 | 0.71 | 0.31263 |
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Target: 5'- cUCAaacUCCCGGAaugauucgAUGCGGCCGcaacaGCGCACg -3' miRNA: 3'- -AGU---AGGGCCU--------UGCGCUGGCc----UGUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 40635 | 0.66 | 0.590246 |
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Target: 5'- --uUCCCGGuccAUGCG-CCGuGAUGCGCu -3' miRNA: 3'- aguAGGGCCu--UGCGCuGGC-CUGUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 41325 | 0.75 | 0.181336 |
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Target: 5'- -gGUUCCGGAGCugaucgGCGACCGGAUcgucaGCGCg -3' miRNA: 3'- agUAGGGCCUUG------CGCUGGCCUG-----UGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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