Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33030 | 5' | -56.5 | NC_007497.1 | + | 32519 | 0.67 | 0.565216 |
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Target: 5'- cCAUCuuGcGAccggcggccacgucACGgGGCCGGGCACGu -3' miRNA: 3'- aGUAGggC-CU--------------UGCgCUGGCCUGUGUg -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 40635 | 0.66 | 0.590246 |
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Target: 5'- --uUCCCGGuccAUGCG-CCGuGAUGCGCu -3' miRNA: 3'- aguAGGGCCu--UGCGCuGGC-CUGUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 31243 | 0.66 | 0.601193 |
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Target: 5'- cCAUCgUCGGaAGCGCcGCCcagcaGGACACGCc -3' miRNA: 3'- aGUAG-GGCC-UUGCGcUGG-----CCUGUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 24016 | 0.66 | 0.601193 |
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Target: 5'- cUCGagCUCGucGCGCGGCCGGACcCGa -3' miRNA: 3'- -AGUa-GGGCcuUGCGCUGGCCUGuGUg -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 2636 | 0.66 | 0.616561 |
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Target: 5'- aCGcgCUCGGAAaucgcaaggaugcgcCGCGGCCGccGGCGCGCa -3' miRNA: 3'- aGUa-GGGCCUU---------------GCGCUGGC--CUGUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 2539 | 0.66 | 0.623156 |
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Target: 5'- ---gCCCGGGcguGCGCGccggcgGCCGcGGCGCAUc -3' miRNA: 3'- aguaGGGCCU---UGCGC------UGGC-CUGUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 26173 | 0.66 | 0.634155 |
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Target: 5'- ----gCCGGGAcCGuCGGCCGGAUAC-Ca -3' miRNA: 3'- aguagGGCCUU-GC-GCUGGCCUGUGuG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 15308 | 0.66 | 0.634155 |
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Target: 5'- gCGUCCgCGGGAUGCG-CCacaGGCGCGg -3' miRNA: 3'- aGUAGG-GCCUUGCGCuGGc--CUGUGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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