Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 33030 | 5' | -56.5 | NC_007497.1 | + | 32832 | 0.69 | 0.42476 |
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Target: 5'- -aGUCcgCCGaGAACGCcGCUGGGCGCGCc -3' miRNA: 3'- agUAG--GGC-CUUGCGcUGGCCUGUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 21697 | 0.69 | 0.415313 |
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Target: 5'- aUC-UUCCGGAACGUGuCCGcgacgagcuGACGCGCu -3' miRNA: 3'- -AGuAGGGCCUUGCGCuGGC---------CUGUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 16234 | 0.7 | 0.370077 |
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Target: 5'- cCGUCUCGGuGCGUG-CCGGGaucaGCGCa -3' miRNA: 3'- aGUAGGGCCuUGCGCuGGCCUg---UGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 1782 | 0.7 | 0.360587 |
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Target: 5'- -gAUCCCcgcuGggUGCGGCCGGgagugcuGCGCACg -3' miRNA: 3'- agUAGGGc---CuuGCGCUGGCC-------UGUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 32656 | 0.71 | 0.344599 |
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Target: 5'- uUCGguuggCUCGGcAAUGCGAUCGGACuugGCGCg -3' miRNA: 3'- -AGUa----GGGCC-UUGCGCUGGCCUG---UGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 24629 | 0.72 | 0.282961 |
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Target: 5'- aCAUCCCgGGAAC-CGGCUGGGCAg-- -3' miRNA: 3'- aGUAGGG-CCUUGcGCUGGCCUGUgug -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 1821 | 0.93 | 0.010314 |
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Target: 5'- uUCAUCCCGGAACG-GcACCGGACACACa -3' miRNA: 3'- -AGUAGGGCCUUGCgC-UGGCCUGUGUG- -5' |
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| 33030 | 5' | -56.5 | NC_007497.1 | + | 15308 | 0.66 | 0.634155 |
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Target: 5'- gCGUCCgCGGGAUGCG-CCacaGGCGCGg -3' miRNA: 3'- aGUAGG-GCCUUGCGCuGGc--CUGUGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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