Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33032 | 5' | -57.3 | NC_007497.1 | + | 2549 | 0.65 | 0.529768 |
Target: 5'- gCGCGCCGGCGgccgcggcgcauccUUGCgauuuccGAGCGCGuagauGCGUg -3' miRNA: 3'- -GCGUGGCCGU--------------GACGa------UUCGCGC-----UGUA- -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 27176 | 0.66 | 0.526534 |
Target: 5'- gCGCAUCGGCggccguauccgcuccGCUGCc-GGCGCGGu-- -3' miRNA: 3'- -GCGUGGCCG---------------UGACGauUCGCGCUgua -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 44552 | 0.66 | 0.522235 |
Target: 5'- gGCGCCGGC--UGCUGAGacugauCGcCGGCAUu -3' miRNA: 3'- gCGUGGCCGugACGAUUC------GC-GCUGUA- -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 31391 | 0.66 | 0.500951 |
Target: 5'- -aUACUGGCACguUGCaGAGCGCGGgAa -3' miRNA: 3'- gcGUGGCCGUG--ACGaUUCGCGCUgUa -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 19872 | 0.66 | 0.489408 |
Target: 5'- uGCACCGcGCugaucugGCUGUUGaucGGUGCGACc- -3' miRNA: 3'- gCGUGGC-CG-------UGACGAU---UCGCGCUGua -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 27095 | 0.66 | 0.480057 |
Target: 5'- cCGCGCCGGCA--GCgGAGCG-GAUAc -3' miRNA: 3'- -GCGUGGCCGUgaCGaUUCGCgCUGUa -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 4193 | 0.67 | 0.469771 |
Target: 5'- cCGCGagCGGUuuaugGCUGCgguGCGCGACGc -3' miRNA: 3'- -GCGUg-GCCG-----UGACGauuCGCGCUGUa -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 2490 | 0.67 | 0.449544 |
Target: 5'- uCGUcCgGGCGgUGCUucuucAGGUGCGACAUg -3' miRNA: 3'- -GCGuGgCCGUgACGA-----UUCGCGCUGUA- -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 5818 | 0.67 | 0.448545 |
Target: 5'- cCGC-CCGGCAgCUucgucgGCUgacgagcGAGCGCGGCGa -3' miRNA: 3'- -GCGuGGCCGU-GA------CGA-------UUCGCGCUGUa -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 38050 | 0.67 | 0.428833 |
Target: 5'- uGCGauacCCGGCGaauccuuCUGCuUGAGCGCGGCc- -3' miRNA: 3'- gCGU----GGCCGU-------GACG-AUUCGCGCUGua -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 24504 | 0.68 | 0.410588 |
Target: 5'- gCGauaACUGGCGCUGCguacGCGCGGgAUu -3' miRNA: 3'- -GCg--UGGCCGUGACGauu-CGCGCUgUA- -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 39275 | 0.68 | 0.404927 |
Target: 5'- gGCGgCGGC-CUGCUGAauccggagccgaugcGCGCGAUg- -3' miRNA: 3'- gCGUgGCCGuGACGAUU---------------CGCGCUGua -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 23189 | 0.68 | 0.401181 |
Target: 5'- uCGCcgGCCaGCAgUGC-GAGCGCGACu- -3' miRNA: 3'- -GCG--UGGcCGUgACGaUUCGCGCUGua -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 473 | 0.68 | 0.401181 |
Target: 5'- gCGCGCUGGCAagaGCagcAGUGCGGCGc -3' miRNA: 3'- -GCGUGGCCGUga-CGau-UCGCGCUGUa -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 36536 | 0.68 | 0.391912 |
Target: 5'- uGaCACUGGCGCUGC--GGCuCGGCAg -3' miRNA: 3'- gC-GUGGCCGUGACGauUCGcGCUGUa -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 3388 | 0.68 | 0.391912 |
Target: 5'- gCGCGCgGGCGauaUGCcuuGCGCGGCu- -3' miRNA: 3'- -GCGUGgCCGUg--ACGauuCGCGCUGua -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 34565 | 0.68 | 0.382784 |
Target: 5'- gGCAuccugacgaccgUCGGCACggugGCgcuuGGCGCGGCAg -3' miRNA: 3'- gCGU------------GGCCGUGa---CGau--UCGCGCUGUa -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 23121 | 0.68 | 0.382784 |
Target: 5'- uCGCGCUcGCACUGCU-GGC-CGGCGa -3' miRNA: 3'- -GCGUGGcCGUGACGAuUCGcGCUGUa -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 41170 | 0.68 | 0.382784 |
Target: 5'- gGCACCGGCgucaccGCgaaGCggaucAGCGCGGCGc -3' miRNA: 3'- gCGUGGCCG------UGa--CGau---UCGCGCUGUa -5' |
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33032 | 5' | -57.3 | NC_007497.1 | + | 8804 | 0.68 | 0.373798 |
Target: 5'- uGCGCCGGUAUcGCUcuAAGCaauguuGCGACGg -3' miRNA: 3'- gCGUGGCCGUGaCGA--UUCG------CGCUGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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