Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33035 | 5' | -56.1 | NC_007497.1 | + | 26853 | 0.66 | 0.553069 |
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Target: 5'- gAUCGaCGAcGGCGCGCagauucagcagcGGCCGGUGa -3' miRNA: 3'- -UAGUaGUUuUCGCGCGa-----------CCGGCCGCg -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 17633 | 0.66 | 0.555277 |
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Target: 5'- -cCGUCAccucGGCGCGCggcuccGGCUGGCcCg -3' miRNA: 3'- uaGUAGUuu--UCGCGCGa-----CCGGCCGcG- -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 35052 | 0.66 | 0.555277 |
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Target: 5'- -aCA-CGGAuGCGaaGCUGGCCgaGGCGCg -3' miRNA: 3'- uaGUaGUUUuCGCg-CGACCGG--CCGCG- -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 2910 | 0.66 | 0.566353 |
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Target: 5'- uUCAacggCAGAAGCaugcacgcgGUGCUGGCgcaUGGUGCg -3' miRNA: 3'- uAGUa---GUUUUCG---------CGCGACCG---GCCGCG- -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 21471 | 0.66 | 0.57414 |
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Target: 5'- cAUCGUCuGAAAGCagggcguccgugacGCGCUGGaUCuGCGCu -3' miRNA: 3'- -UAGUAG-UUUUCG--------------CGCGACC-GGcCGCG- -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 15323 | 0.66 | 0.577485 |
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Target: 5'- ---------cGCgGCGCUcGGCCGGCGUc -3' miRNA: 3'- uaguaguuuuCG-CGCGA-CCGGCCGCG- -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 992 | 0.66 | 0.585306 |
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Target: 5'- gAUCAUCGAAGGCgucuucggggugaaGCGCagccUGGCUGaCGUg -3' miRNA: 3'- -UAGUAGUUUUCG--------------CGCG----ACCGGCcGCG- -5' |
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| 33035 | 5' | -56.1 | NC_007497.1 | + | 3812 | 0.66 | 0.588665 |
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Target: 5'- cUCG-CAu--GCagGCGCUcggucgacugaaGGCCGGCGCg -3' miRNA: 3'- uAGUaGUuuuCG--CGCGA------------CCGGCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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