Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33047 | 3' | -53.9 | NC_007497.1 | + | 21552 | 0.71 | 0.405165 |
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Target: 5'- uGCGGcAGAGGCgUGGGCGCAGCUcGUCg -3' miRNA: 3'- gCGUC-UCUUUG-GUCUGCGUCGGcUAG- -5' |
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| 33047 | 3' | -53.9 | NC_007497.1 | + | 14447 | 0.72 | 0.359346 |
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Target: 5'- cCGCAGGG----CGGGCGCGGuuGAUCa -3' miRNA: 3'- -GCGUCUCuuugGUCUGCGUCggCUAG- -5' |
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| 33047 | 3' | -53.9 | NC_007497.1 | + | 9803 | 0.72 | 0.359346 |
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Target: 5'- aGUAGAuaucGAuAACCuGcCGCAGCCGAUCg -3' miRNA: 3'- gCGUCU----CU-UUGGuCuGCGUCGGCUAG- -5' |
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| 33047 | 3' | -53.9 | NC_007497.1 | + | 32939 | 0.73 | 0.325382 |
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Target: 5'- gCGCGGAGGccGACCAGcucgaacgugaACGCGGCuaucuCGAUCa -3' miRNA: 3'- -GCGUCUCU--UUGGUC-----------UGCGUCG-----GCUAG- -5' |
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| 33047 | 3' | -53.9 | NC_007497.1 | + | 12114 | 0.75 | 0.244525 |
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Target: 5'- cCGCAuucGAGu-ACUcGGCGCGGCCGAUCa -3' miRNA: 3'- -GCGU---CUCuuUGGuCUGCGUCGGCUAG- -5' |
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| 33047 | 3' | -53.9 | NC_007497.1 | + | 15560 | 1.11 | 0.000753 |
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Target: 5'- gCGCAGAGAAACCAGACGCAGCCGAUCg -3' miRNA: 3'- -GCGUCUCUUUGGUCUGCGUCGGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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