Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
33064 | 5' | -59 | NC_007497.1 | + | 15917 | 0.66 | 0.418229 |
Target: 5'- aGGGUGgGUGcUCGGCucGCauaUGGCAGCGUu -3' miRNA: 3'- -UCCGCgCAC-AGCUG--CG---ACUGUCGCGu -5' |
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33064 | 5' | -59 | NC_007497.1 | + | 31325 | 0.66 | 0.437234 |
Target: 5'- cGGUggGCGUGUCc-UGCUgGGCGGCGCu -3' miRNA: 3'- uCCG--CGCACAGcuGCGA-CUGUCGCGu -5' |
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33064 | 5' | -59 | NC_007497.1 | + | 40816 | 0.66 | 0.44692 |
Target: 5'- cGGCGCGag-CGuCGCUG-CAGCGg- -3' miRNA: 3'- uCCGCGCacaGCuGCGACuGUCGCgu -5' |
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33064 | 5' | -59 | NC_007497.1 | + | 33439 | 0.66 | 0.44692 |
Target: 5'- cAGGCGCa--UUGACGCcGAUAcGCGCGa -3' miRNA: 3'- -UCCGCGcacAGCUGCGaCUGU-CGCGU- -5' |
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33064 | 5' | -59 | NC_007497.1 | + | 40558 | 0.66 | 0.466644 |
Target: 5'- cGGGCGC-UGgcgaaGACGUUG-CAGCGUc -3' miRNA: 3'- -UCCGCGcACag---CUGCGACuGUCGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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