miRNA: 3'- -GUCGAGCGACuAGCGG-CUGU--------GCCacu -5'
miRNA: 3'- -GUCGAg----C-GACUAGCGGCUGU-GCCacu -5'
miRNA: 3'- guCGaGCGACUAGCGGCUg------UGCCacu -5'
miRNA: 3'- guCGagCGACUAGCGGCUG-UGCCacu -5'
miRNA: 3'- gUCGaGCGACuAGCG-GCUGUGCcACU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33068 | 3' | -56.7 | NC_007497.1 | + | 21345 | 0.66 | 0.563947 |
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Target: 5'- uCAGCUCGCUG-UCGCUaGACAgcgagcauCGGa-- -3' miRNA: 3'- -GUCGAGCGACuAGCGG-CUGU--------GCCacu -5' |
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| 33068 | 3' | -56.7 | NC_007497.1 | + | 44556 | 0.66 | 0.574856 |
|
Target: 5'- cCGGCUgcugaGaCUGAUCGCCGGCAuUGGa-- -3' miRNA: 3'- -GUCGAg----C-GACUAGCGGCUGU-GCCacu -5' |
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| 33068 | 3' | -56.7 | NC_007497.1 | + | 21693 | 0.66 | 0.585812 |
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Target: 5'- -cGC-CGCUGAUCGCUGGaaacgcaGCGGa-- -3' miRNA: 3'- guCGaGCGACUAGCGGCUg------UGCCacu -5' |
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| 33068 | 3' | -56.7 | NC_007497.1 | + | 8530 | 0.66 | 0.596807 |
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Target: 5'- -uGCg-GCUGAUCGUCGGCuGCGGa-- -3' miRNA: 3'- guCGagCGACUAGCGGCUG-UGCCacu -5' |
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| 33068 | 3' | -56.7 | NC_007497.1 | + | 41526 | 0.66 | 0.596807 |
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Target: 5'- gGGCgCGCUG-UUGCgCGGCACGcUGAa -3' miRNA: 3'- gUCGaGCGACuAGCG-GCUGUGCcACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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