Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33273 | 5' | -45 | NC_007605.1 | + | 7443 | 0.67 | 0.999981 |
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Target: 5'- aGGAuAGCAUAUGCUAccCGGAUacagauUAGGa- -3' miRNA: 3'- -CCU-UCGUAUACGAUa-GCUUA------AUCCca -5' |
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| 33273 | 5' | -45 | NC_007605.1 | + | 108053 | 0.67 | 0.999981 |
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Target: 5'- uGGggGUggGUGCaa--GAAUUGGGGc -3' miRNA: 3'- -CCuuCGuaUACGauagCUUAAUCCCa -5' |
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| 33273 | 5' | -45 | NC_007605.1 | + | 7769 | 0.67 | 0.999981 |
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Target: 5'- aGGAuAGCAUAUGCUAccCGGAUacagauUAGGa- -3' miRNA: 3'- -CCU-UCGUAUACGAUa-GCUUA------AUCCca -5' |
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| 33273 | 5' | -45 | NC_007605.1 | + | 119498 | 0.66 | 0.999996 |
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Target: 5'- uGGggGCcaagGUGCU-UCGGGguaaggggcgugauuUUGGGGUg -3' miRNA: 3'- -CCuuCGua--UACGAuAGCUU---------------AAUCCCA- -5' |
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| 33273 | 5' | -45 | NC_007605.1 | + | 169281 | 0.66 | 0.999998 |
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Target: 5'- uGAGGCAag-GCUGUgGggUaaccgUAGGGg -3' miRNA: 3'- cCUUCGUauaCGAUAgCuuA-----AUCCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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