miRNA: 3'- -CCU-UCGUAUACGAUAGcu---UAAuCCCA- -5'
miRNA: 3'- -CCU-UCGUAUACGAUAGc-------UUAAUCCCA- -5'
miRNA: 3'- -CCUUCGUAUACGAUa-GCuuAAUCCCA- -5'
miRNA: 3'- -CCUuc-GUAUACGAUAGCUUAAUCCCA- -5'
miRNA: 3'- -CCUUCGUAUACGAUAGCUUAAUCCCA- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 33273 | 5' | -45 | NC_007605.1 | + | 7683 | 0.75 | 0.982227 |
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Target: 5'- aGGAuAGCAUAUGCUAUCcagauAUUuGGGUa -3' miRNA: 3'- -CCU-UCGUAUACGAUAGcu---UAAuCCCA- -5' |
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| 33273 | 5' | -45 | NC_007605.1 | + | 7959 | 0.82 | 0.776189 |
|
Target: 5'- cGGGuAGCAUAUGCUAUCcuaauagaGAUUAGGGUa -3' miRNA: 3'- -CCU-UCGUAUACGAUAGc-------UUAAUCCCA- -5' |
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| 33273 | 5' | -45 | NC_007605.1 | + | 9098 | 0.83 | 0.713575 |
|
Target: 5'- gGGAAGCAUAUGCUAccCGu-UUAGGGUu -3' miRNA: 3'- -CCUUCGUAUACGAUa-GCuuAAUCCCA- -5' |
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| 33273 | 5' | -45 | NC_007605.1 | + | 9043 | 0.85 | 0.614936 |
|
Target: 5'- uGGGuaaCAUAUGCUAUUGAAUUAGGGUu -3' miRNA: 3'- -CCUuc-GUAUACGAUAGCUUAAUCCCA- -5' |
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| 33273 | 5' | -45 | NC_007605.1 | + | 9211 | 1.11 | 0.029805 |
|
Target: 5'- gGGAAGCAUAUGCUAUCGAAUUAGGGUu -3' miRNA: 3'- -CCUUCGUAUACGAUAGCUUAAUCCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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