Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3504 | 3' | -42.7 | NC_001623.1 | + | 9839 | 0.66 | 1 |
Target: 5'- aGAaaaauAGCuAAACGUGUAAUUGACg -3' miRNA: 3'- aCUacguuUCG-UUUGUACAUUAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 122808 | 0.66 | 1 |
Target: 5'- cGgcGCGcAAGCGAGCGUGUGcgccggagcacaAUUGAUa -3' miRNA: 3'- aCuaCGU-UUCGUUUGUACAU------------UAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 13702 | 0.66 | 1 |
Target: 5'- -cGUGCuuGGCAAAaCAcGUAcUCGGCg -3' miRNA: 3'- acUACGuuUCGUUU-GUaCAUuAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 85066 | 0.66 | 1 |
Target: 5'- ---gGCAGAucGUcGACAagUGUGAUCGACu -3' miRNA: 3'- acuaCGUUU--CGuUUGU--ACAUUAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 49690 | 0.66 | 1 |
Target: 5'- --uUGCAGAGCAaaAACggGUAcgCGGu -3' miRNA: 3'- acuACGUUUCGU--UUGuaCAUuaGCUg -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 27349 | 0.66 | 1 |
Target: 5'- cGAUGCGAuuucugucaacGGCAAcgcgcuugaAUAUGguGUCGACg -3' miRNA: 3'- aCUACGUU-----------UCGUU---------UGUACauUAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 112599 | 0.67 | 0.999999 |
Target: 5'- aGGUcaaCGAcGCAAACAUGauGUCGACg -3' miRNA: 3'- aCUAc--GUUuCGUUUGUACauUAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 106917 | 0.67 | 0.999999 |
Target: 5'- ---cGCGAuucccGGCAcGCcgGUGAUCGAUu -3' miRNA: 3'- acuaCGUU-----UCGUuUGuaCAUUAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 40429 | 0.67 | 0.999999 |
Target: 5'- cGAUGCGagcuuuuauucaaccGAGCGuGCAUGUuugcAAUCGuGCa -3' miRNA: 3'- aCUACGU---------------UUCGUuUGUACA----UUAGC-UG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 33217 | 0.67 | 0.999998 |
Target: 5'- ---cGCGcuuuacaaaacGAGguAGCGUGUAAUCGAa -3' miRNA: 3'- acuaCGU-----------UUCguUUGUACAUUAGCUg -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 109413 | 0.67 | 0.999998 |
Target: 5'- -aGUGCGAGGUggGCGaagagcUGAUCGACc -3' miRNA: 3'- acUACGUUUCGuuUGUac----AUUAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 68361 | 0.68 | 0.999997 |
Target: 5'- -uAUGCGGGGCAuuuCAUGgucacGUCGGCc -3' miRNA: 3'- acUACGUUUCGUuu-GUACau---UAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 53977 | 0.68 | 0.999989 |
Target: 5'- cGAUGCAAuauacauuGCGAAUAUacagAAUCGGCc -3' miRNA: 3'- aCUACGUUu-------CGUUUGUAca--UUAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 44203 | 0.68 | 0.999989 |
Target: 5'- cGA-GCAuuGCAAACAuauUGUGGaCGGCg -3' miRNA: 3'- aCUaCGUuuCGUUUGU---ACAUUaGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 128163 | 0.69 | 0.999985 |
Target: 5'- aUGAaGuCAGAGCAgcuuuaAACGUGUGgugcacAUCGACg -3' miRNA: 3'- -ACUaC-GUUUCGU------UUGUACAU------UAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 59591 | 0.69 | 0.99997 |
Target: 5'- --uUGCAAAGCGgguucaacGACAUGguaUAGUUGGCa -3' miRNA: 3'- acuACGUUUCGU--------UUGUAC---AUUAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 120532 | 0.69 | 0.99997 |
Target: 5'- aGAUGCAuuuguuGuCGAGCGUGUAucuaaacUCGGCa -3' miRNA: 3'- aCUACGUuu----C-GUUUGUACAUu------AGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 34977 | 0.7 | 0.999899 |
Target: 5'- aUGAgcaGCAAGuGCAAACGaGUGcUCGACu -3' miRNA: 3'- -ACUa--CGUUU-CGUUUGUaCAUuAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 41415 | 0.7 | 0.999866 |
Target: 5'- --uUGaAGGGCAAGCuUGUGGUCGGCc -3' miRNA: 3'- acuACgUUUCGUUUGuACAUUAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 32335 | 0.7 | 0.999866 |
Target: 5'- cGGUGCAcauuacGGCAAACGUGUuGUCa-- -3' miRNA: 3'- aCUACGUu-----UCGUUUGUACAuUAGcug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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