Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3504 | 3' | -42.7 | NC_001623.1 | + | 51139 | 0.71 | 0.999702 |
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Target: 5'- aGAuauUGCAAAGCAAAC-UGUAcuucuUUGGCg -3' miRNA: 3'- aCU---ACGUUUCGUUUGuACAUu----AGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 120816 | 0.71 | 0.999702 |
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Target: 5'- uUGAcGCGGGacGC-AGCGUGUAGUCGAUu -3' miRNA: 3'- -ACUaCGUUU--CGuUUGUACAUUAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 131398 | 0.72 | 0.999383 |
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Target: 5'- cUGcgGCGAGGCAAACAaaucuggcgacUGUGGuauuacguaauUCGGCg -3' miRNA: 3'- -ACuaCGUUUCGUUUGU-----------ACAUU-----------AGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 35826 | 0.72 | 0.999225 |
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Target: 5'- cGGUGCGgcGCuacAACAgacgcGUAAUCGACu -3' miRNA: 3'- aCUACGUuuCGu--UUGUa----CAUUAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 108489 | 0.73 | 0.998527 |
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Target: 5'- uUGGUGCAcGGCAucagcaaaAACGUGUcGUcCGACa -3' miRNA: 3'- -ACUACGUuUCGU--------UUGUACAuUA-GCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 31492 | 0.74 | 0.996208 |
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Target: 5'- --uUGCAcAGCAAAUGUGgguuGUCGACa -3' miRNA: 3'- acuACGUuUCGUUUGUACau--UAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 6852 | 0.76 | 0.98695 |
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Target: 5'- uUGAcaaCAAAGCAuACAUGUAcGUCGACg -3' miRNA: 3'- -ACUac-GUUUCGUuUGUACAU-UAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 25901 | 1.14 | 0.02675 |
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Target: 5'- uUGAUGCAAAGCAAACAUGUAAUCGACg -3' miRNA: 3'- -ACUACGUUUCGUUUGUACAUUAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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