Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 3504 | 3' | -42.7 | NC_001623.1 | + | 108489 | 0.73 | 0.998527 |
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Target: 5'- uUGGUGCAcGGCAucagcaaaAACGUGUcGUcCGACa -3' miRNA: 3'- -ACUACGUuUCGU--------UUGUACAuUA-GCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 109413 | 0.67 | 0.999998 |
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Target: 5'- -aGUGCGAGGUggGCGaagagcUGAUCGACc -3' miRNA: 3'- acUACGUUUCGuuUGUac----AUUAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 112599 | 0.67 | 0.999999 |
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Target: 5'- aGGUcaaCGAcGCAAACAUGauGUCGACg -3' miRNA: 3'- aCUAc--GUUuCGUUUGUACauUAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 120532 | 0.69 | 0.99997 |
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Target: 5'- aGAUGCAuuuguuGuCGAGCGUGUAucuaaacUCGGCa -3' miRNA: 3'- aCUACGUuu----C-GUUUGUACAUu------AGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 120816 | 0.71 | 0.999702 |
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Target: 5'- uUGAcGCGGGacGC-AGCGUGUAGUCGAUu -3' miRNA: 3'- -ACUaCGUUU--CGuUUGUACAUUAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 122808 | 0.66 | 1 |
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Target: 5'- cGgcGCGcAAGCGAGCGUGUGcgccggagcacaAUUGAUa -3' miRNA: 3'- aCuaCGU-UUCGUUUGUACAU------------UAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 128163 | 0.69 | 0.999985 |
|
Target: 5'- aUGAaGuCAGAGCAgcuuuaAACGUGUGgugcacAUCGACg -3' miRNA: 3'- -ACUaC-GUUUCGU------UUGUACAU------UAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 131398 | 0.72 | 0.999383 |
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Target: 5'- cUGcgGCGAGGCAAACAaaucuggcgacUGUGGuauuacguaauUCGGCg -3' miRNA: 3'- -ACuaCGUUUCGUUUGU-----------ACAUU-----------AGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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