Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3504 | 3' | -42.7 | NC_001623.1 | + | 112599 | 0.67 | 0.999999 |
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Target: 5'- aGGUcaaCGAcGCAAACAUGauGUCGACg -3' miRNA: 3'- aCUAc--GUUuCGUUUGUACauUAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 106917 | 0.67 | 0.999999 |
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Target: 5'- ---cGCGAuucccGGCAcGCcgGUGAUCGAUu -3' miRNA: 3'- acuaCGUU-----UCGUuUGuaCAUUAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 122808 | 0.66 | 1 |
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Target: 5'- cGgcGCGcAAGCGAGCGUGUGcgccggagcacaAUUGAUa -3' miRNA: 3'- aCuaCGU-UUCGUUUGUACAU------------UAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 13702 | 0.66 | 1 |
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Target: 5'- -cGUGCuuGGCAAAaCAcGUAcUCGGCg -3' miRNA: 3'- acUACGuuUCGUUU-GUaCAUuAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 85066 | 0.66 | 1 |
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Target: 5'- ---gGCAGAucGUcGACAagUGUGAUCGACu -3' miRNA: 3'- acuaCGUUU--CGuUUGU--ACAUUAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 27349 | 0.66 | 1 |
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Target: 5'- cGAUGCGAuuucugucaacGGCAAcgcgcuugaAUAUGguGUCGACg -3' miRNA: 3'- aCUACGUU-----------UCGUU---------UGUACauUAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 49690 | 0.66 | 1 |
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Target: 5'- --uUGCAGAGCAaaAACggGUAcgCGGu -3' miRNA: 3'- acuACGUUUCGU--UUGuaCAUuaGCUg -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 9839 | 0.66 | 1 |
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Target: 5'- aGAaaaauAGCuAAACGUGUAAUUGACg -3' miRNA: 3'- aCUacguuUCG-UUUGUACAUUAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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