Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 3504 | 3' | -42.7 | NC_001623.1 | + | 128163 | 0.69 | 0.999985 |
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Target: 5'- aUGAaGuCAGAGCAgcuuuaAACGUGUGgugcacAUCGACg -3' miRNA: 3'- -ACUaC-GUUUCGU------UUGUACAU------UAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 59591 | 0.69 | 0.99997 |
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Target: 5'- --uUGCAAAGCGgguucaacGACAUGguaUAGUUGGCa -3' miRNA: 3'- acuACGUUUCGU--------UUGUAC---AUUAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 32335 | 0.7 | 0.999866 |
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Target: 5'- cGGUGCAcauuacGGCAAACGUGUuGUCa-- -3' miRNA: 3'- aCUACGUu-----UCGUUUGUACAuUAGcug -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 41415 | 0.7 | 0.999866 |
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Target: 5'- --uUGaAGGGCAAGCuUGUGGUCGGCc -3' miRNA: 3'- acuACgUUUCGUUUGuACAUUAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 51139 | 0.71 | 0.999702 |
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Target: 5'- aGAuauUGCAAAGCAAAC-UGUAcuucuUUGGCg -3' miRNA: 3'- aCU---ACGUUUCGUUUGuACAUu----AGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 6852 | 0.76 | 0.98695 |
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Target: 5'- uUGAcaaCAAAGCAuACAUGUAcGUCGACg -3' miRNA: 3'- -ACUac-GUUUCGUuUGUACAU-UAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 25901 | 1.14 | 0.02675 |
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Target: 5'- uUGAUGCAAAGCAAACAUGUAAUCGACg -3' miRNA: 3'- -ACUACGUUUCGUUUGUACAUUAGCUG- -5' |
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| 3504 | 3' | -42.7 | NC_001623.1 | + | 9839 | 0.66 | 1 |
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Target: 5'- aGAaaaauAGCuAAACGUGUAAUUGACg -3' miRNA: 3'- aCUacguuUCG-UUUGUACAUUAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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