Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3504 | 3' | -42.7 | NC_001623.1 | + | 33217 | 0.67 | 0.999998 |
Target: 5'- ---cGCGcuuuacaaaacGAGguAGCGUGUAAUCGAa -3' miRNA: 3'- acuaCGU-----------UUCguUUGUACAUUAGCUg -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 32335 | 0.7 | 0.999866 |
Target: 5'- cGGUGCAcauuacGGCAAACGUGUuGUCa-- -3' miRNA: 3'- aCUACGUu-----UCGUUUGUACAuUAGcug -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 31492 | 0.74 | 0.996208 |
Target: 5'- --uUGCAcAGCAAAUGUGgguuGUCGACa -3' miRNA: 3'- acuACGUuUCGUUUGUACau--UAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 27349 | 0.66 | 1 |
Target: 5'- cGAUGCGAuuucugucaacGGCAAcgcgcuugaAUAUGguGUCGACg -3' miRNA: 3'- aCUACGUU-----------UCGUU---------UGUACauUAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 25901 | 1.14 | 0.02675 |
Target: 5'- uUGAUGCAAAGCAAACAUGUAAUCGACg -3' miRNA: 3'- -ACUACGUUUCGUUUGUACAUUAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 13702 | 0.66 | 1 |
Target: 5'- -cGUGCuuGGCAAAaCAcGUAcUCGGCg -3' miRNA: 3'- acUACGuuUCGUUU-GUaCAUuAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 9839 | 0.66 | 1 |
Target: 5'- aGAaaaauAGCuAAACGUGUAAUUGACg -3' miRNA: 3'- aCUacguuUCG-UUUGUACAUUAGCUG- -5' |
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3504 | 3' | -42.7 | NC_001623.1 | + | 6852 | 0.76 | 0.98695 |
Target: 5'- uUGAcaaCAAAGCAuACAUGUAcGUCGACg -3' miRNA: 3'- -ACUac-GUUUCGUuUGUACAU-UAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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