Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3507 | 5' | -53.1 | NC_001623.1 | + | 32027 | 0.66 | 0.968002 |
Target: 5'- --aGGCAGGAgCgUCGGCGUuAACGa -3' miRNA: 3'- cggUCGUUCUgGaAGCCGCGuUUGCg -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 61617 | 0.66 | 0.968002 |
Target: 5'- uGUguGCAcGGAauguUCgcgUCGGUGCAAGCGUu -3' miRNA: 3'- -CGguCGU-UCU----GGa--AGCCGCGUUUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 6397 | 0.66 | 0.967686 |
Target: 5'- uUCGcGCAcgauuguGGGCac-CGGCGCAGGCGCc -3' miRNA: 3'- cGGU-CGU-------UCUGgaaGCCGCGUUUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 65797 | 0.66 | 0.96406 |
Target: 5'- cGCCAGCucGGCCauguaauugaauaugUUggcagacgcagauagCGGCGCcaaAAACGCa -3' miRNA: 3'- -CGGUCGuuCUGG---------------AA---------------GCCGCG---UUUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 14651 | 0.66 | 0.961251 |
Target: 5'- cGCCGGCAGG----UUGGCaaaCAAGCGCu -3' miRNA: 3'- -CGGUCGUUCuggaAGCCGc--GUUUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 87646 | 0.66 | 0.957531 |
Target: 5'- -gCGGCGuAGGCUgcgCGGaCGCuGGCGCg -3' miRNA: 3'- cgGUCGU-UCUGGaa-GCC-GCGuUUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 22159 | 0.66 | 0.957531 |
Target: 5'- aCCAGCAgcuGGuGCCguacaacagCGGCGCuuuuAACGUg -3' miRNA: 3'- cGGUCGU---UC-UGGaa-------GCCGCGu---UUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 77487 | 0.66 | 0.957531 |
Target: 5'- -aCAGUAcGAaug-CGGCGUGAACGCg -3' miRNA: 3'- cgGUCGUuCUggaaGCCGCGUUUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 109954 | 0.66 | 0.953573 |
Target: 5'- uUCGGCcguguGGACCaacgCGGCGCccuCGCa -3' miRNA: 3'- cGGUCGu----UCUGGaa--GCCGCGuuuGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 75485 | 0.67 | 0.949374 |
Target: 5'- cGCCuGCAAcACCa--GGCGCAGgaccugccacuGCGCc -3' miRNA: 3'- -CGGuCGUUcUGGaagCCGCGUU-----------UGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 119918 | 0.67 | 0.949374 |
Target: 5'- uGCuUAGCAcGAC--UCGGCGCGugaccguGCGCa -3' miRNA: 3'- -CG-GUCGUuCUGgaAGCCGCGUu------UGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 120563 | 0.67 | 0.940237 |
Target: 5'- cUCGGC-AGACUgaacuuggguUUCGGCGCcuucACGCa -3' miRNA: 3'- cGGUCGuUCUGG----------AAGCCGCGuu--UGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 106442 | 0.67 | 0.930099 |
Target: 5'- uGCCAGau-GGCCgccgUCGGUGUcuGCGa -3' miRNA: 3'- -CGGUCguuCUGGa---AGCCGCGuuUGCg -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 61499 | 0.68 | 0.918952 |
Target: 5'- gGCUaAGCGuuaaACCUUCGGCGCcgccCGCc -3' miRNA: 3'- -CGG-UCGUuc--UGGAAGCCGCGuuu-GCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 2148 | 0.68 | 0.912999 |
Target: 5'- -gCAGaCGGcGCCggucUCGGCGCAAGCGg -3' miRNA: 3'- cgGUC-GUUcUGGa---AGCCGCGUUUGCg -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 31083 | 0.68 | 0.902955 |
Target: 5'- aGCCGGaCAAGgcuguccugcuguguGCCcgUCGcgcguaccggaGCGCGAACGCg -3' miRNA: 3'- -CGGUC-GUUC---------------UGGa-AGC-----------CGCGUUUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 75922 | 0.68 | 0.898361 |
Target: 5'- cGCCAGCAccGCCUcgaagcgcUCgGGCGUuggucacgucaaagAAACGCa -3' miRNA: 3'- -CGGUCGUucUGGA--------AG-CCGCG--------------UUUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 122631 | 0.69 | 0.87212 |
Target: 5'- aUCAGUAucaauuGugCUcCGGCGCAcACGCu -3' miRNA: 3'- cGGUCGUu-----CugGAaGCCGCGUuUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 6037 | 0.71 | 0.757768 |
Target: 5'- cGCaGGCGGGGCUggCGGCGgAGGCGg -3' miRNA: 3'- -CGgUCGUUCUGGaaGCCGCgUUUGCg -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 6570 | 0.71 | 0.757768 |
Target: 5'- aGCCAGCGGcGCCUgcgcCGGUGCccacaaucguGCGCg -3' miRNA: 3'- -CGGUCGUUcUGGAa---GCCGCGuu--------UGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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