Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3507 | 5' | -53.1 | NC_001623.1 | + | 18781 | 0.73 | 0.697057 |
Target: 5'- gGCCuGCGcuAUCUucuaUCGGCGUAAACGUg -3' miRNA: 3'- -CGGuCGUucUGGA----AGCCGCGUUUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 125727 | 0.73 | 0.686679 |
Target: 5'- -aUAGcCAAGACgUUUGGCGCcuuAAGCGCa -3' miRNA: 3'- cgGUC-GUUCUGgAAGCCGCG---UUUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 65716 | 0.73 | 0.676254 |
Target: 5'- gGCCGGCGAauuGACCgcgUUGGCgGCcGACGUu -3' miRNA: 3'- -CGGUCGUU---CUGGa--AGCCG-CGuUUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 10524 | 0.73 | 0.66579 |
Target: 5'- cGUCuGCAuugaaccGAUCUUCGGCGgCGAugGCg -3' miRNA: 3'- -CGGuCGUu------CUGGAAGCCGC-GUUugCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 94831 | 0.73 | 0.655297 |
Target: 5'- aGCCGaauuuuuuuGUAGGGCUcugcuuuaUUCGGCGCAGACGa -3' miRNA: 3'- -CGGU---------CGUUCUGG--------AAGCCGCGUUUGCg -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 78950 | 0.74 | 0.634263 |
Target: 5'- aCCAGCAAGAaacggcaGGCGCAGAUGUu -3' miRNA: 3'- cGGUCGUUCUggaag--CCGCGUUUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 88360 | 1.15 | 0.001804 |
Target: 5'- cGCCAGCAAGACCUUCGGCGCAAACGCa -3' miRNA: 3'- -CGGUCGUUCUGGAAGCCGCGUUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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