Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3507 | 5' | -53.1 | NC_001623.1 | + | 94831 | 0.73 | 0.655297 |
Target: 5'- aGCCGaauuuuuuuGUAGGGCUcugcuuuaUUCGGCGCAGACGa -3' miRNA: 3'- -CGGU---------CGUUCUGG--------AAGCCGCGUUUGCg -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 106442 | 0.67 | 0.930099 |
Target: 5'- uGCCAGau-GGCCgccgUCGGUGUcuGCGa -3' miRNA: 3'- -CGGUCguuCUGGa---AGCCGCGuuUGCg -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 109954 | 0.66 | 0.953573 |
Target: 5'- uUCGGCcguguGGACCaacgCGGCGCccuCGCa -3' miRNA: 3'- cGGUCGu----UCUGGaa--GCCGCGuuuGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 119918 | 0.67 | 0.949374 |
Target: 5'- uGCuUAGCAcGAC--UCGGCGCGugaccguGCGCa -3' miRNA: 3'- -CG-GUCGUuCUGgaAGCCGCGUu------UGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 120563 | 0.67 | 0.940237 |
Target: 5'- cUCGGC-AGACUgaacuuggguUUCGGCGCcuucACGCa -3' miRNA: 3'- cGGUCGuUCUGG----------AAGCCGCGuu--UGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 122631 | 0.69 | 0.87212 |
Target: 5'- aUCAGUAucaauuGugCUcCGGCGCAcACGCu -3' miRNA: 3'- cGGUCGUu-----CugGAaGCCGCGUuUGCG- -5' |
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3507 | 5' | -53.1 | NC_001623.1 | + | 125727 | 0.73 | 0.686679 |
Target: 5'- -aUAGcCAAGACgUUUGGCGCcuuAAGCGCa -3' miRNA: 3'- cgGUC-GUUCUGgAAGCCGCG---UUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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