Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 3507 | 5' | -53.1 | NC_001623.1 | + | 119918 | 0.67 | 0.949374 |
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Target: 5'- uGCuUAGCAcGAC--UCGGCGCGugaccguGCGCa -3' miRNA: 3'- -CG-GUCGUuCUGgaAGCCGCGUu------UGCG- -5' |
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| 3507 | 5' | -53.1 | NC_001623.1 | + | 31083 | 0.68 | 0.902955 |
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Target: 5'- aGCCGGaCAAGgcuguccugcuguguGCCcgUCGcgcguaccggaGCGCGAACGCg -3' miRNA: 3'- -CGGUC-GUUC---------------UGGa-AGC-----------CGCGUUUGCG- -5' |
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| 3507 | 5' | -53.1 | NC_001623.1 | + | 6570 | 0.71 | 0.757768 |
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Target: 5'- aGCCAGCGGcGCCUgcgcCGGUGCccacaaucguGCGCg -3' miRNA: 3'- -CGGUCGUUcUGGAa---GCCGCGuu--------UGCG- -5' |
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| 3507 | 5' | -53.1 | NC_001623.1 | + | 18781 | 0.73 | 0.697057 |
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Target: 5'- gGCCuGCGcuAUCUucuaUCGGCGUAAACGUg -3' miRNA: 3'- -CGGuCGUucUGGA----AGCCGCGUUUGCG- -5' |
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| 3507 | 5' | -53.1 | NC_001623.1 | + | 78950 | 0.74 | 0.634263 |
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Target: 5'- aCCAGCAAGAaacggcaGGCGCAGAUGUu -3' miRNA: 3'- cGGUCGUUCUggaag--CCGCGUUUGCG- -5' |
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| 3507 | 5' | -53.1 | NC_001623.1 | + | 88360 | 1.15 | 0.001804 |
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Target: 5'- cGCCAGCAAGACCUUCGGCGCAAACGCa -3' miRNA: 3'- -CGGUCGUUCUGGAAGCCGCGUUUGCG- -5' |
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| 3507 | 5' | -53.1 | NC_001623.1 | + | 32027 | 0.66 | 0.968002 |
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Target: 5'- --aGGCAGGAgCgUCGGCGUuAACGa -3' miRNA: 3'- cggUCGUUCUgGaAGCCGCGuUUGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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