Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3512 | 5' | -43.6 | NC_001623.1 | + | 122383 | 0.66 | 1 |
Target: 5'- uCCGGccaagccGUUGgaGC-ACAGCAGCAUUg -3' miRNA: 3'- -GGUCau-----UAACgaUGuUGUUGUCGUAG- -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 20432 | 0.66 | 0.999998 |
Target: 5'- aUAGgaucAUUGCaGCGACAAUAGCGa- -3' miRNA: 3'- gGUCau--UAACGaUGUUGUUGUCGUag -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 108480 | 0.66 | 0.999998 |
Target: 5'- gCCGGcgGAUUGgUGCAcgGCAuCAGCAa- -3' miRNA: 3'- -GGUCa-UUAACgAUGU--UGUuGUCGUag -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 100266 | 0.66 | 0.999998 |
Target: 5'- aUAGUAAauaGCUGCAAUuuuGAUAGCGUa -3' miRNA: 3'- gGUCAUUaa-CGAUGUUG---UUGUCGUAg -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 98594 | 0.66 | 0.999998 |
Target: 5'- uCCAGgguuuacuGUUGCgugacuCAACGACGGCcgUg -3' miRNA: 3'- -GGUCau------UAACGau----GUUGUUGUCGuaG- -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 81047 | 0.66 | 0.999998 |
Target: 5'- uUAGUAcgUGCgACAaaaacaaacGCAcCGGCGUCa -3' miRNA: 3'- gGUCAUuaACGaUGU---------UGUuGUCGUAG- -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 92824 | 0.66 | 0.999998 |
Target: 5'- aCAcGUAAUUgaacaugaGCUGCAACAAUccuuuGGCAUUg -3' miRNA: 3'- gGU-CAUUAA--------CGAUGUUGUUG-----UCGUAG- -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 101142 | 0.66 | 0.999998 |
Target: 5'- uCCGGUGcgUG-UGCGACGACGGg--- -3' miRNA: 3'- -GGUCAUuaACgAUGUUGUUGUCguag -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 43260 | 0.67 | 0.999996 |
Target: 5'- gCAGUAAUcGCUucACGAUGACGGaCGUg -3' miRNA: 3'- gGUCAUUAaCGA--UGUUGUUGUC-GUAg -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 66746 | 0.67 | 0.999995 |
Target: 5'- aCAGgaugGAUUacacgaGCcGCAGCAACAGCAc- -3' miRNA: 3'- gGUCa---UUAA------CGaUGUUGUUGUCGUag -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 94950 | 0.67 | 0.999992 |
Target: 5'- aCAGc-AUUGCUAuCAGCuccAGCGGCAUUg -3' miRNA: 3'- gGUCauUAACGAU-GUUG---UUGUCGUAG- -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 71477 | 0.67 | 0.999989 |
Target: 5'- uCCGGcu-UUGCUACAaagaaaagugccGCAgcGCAGCGUg -3' miRNA: 3'- -GGUCauuAACGAUGU------------UGU--UGUCGUAg -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 55125 | 0.67 | 0.999989 |
Target: 5'- uCCGuc--UUGCcGCAACAAguGCAUCa -3' miRNA: 3'- -GGUcauuAACGaUGUUGUUguCGUAG- -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 12805 | 0.68 | 0.999984 |
Target: 5'- aCUAGUAGUUgaucGCUggaaACggUAACAGUGUCc -3' miRNA: 3'- -GGUCAUUAA----CGA----UGuuGUUGUCGUAG- -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 131534 | 0.68 | 0.999984 |
Target: 5'- gCAGUcuccgcaGCcGCAGCAGCAGCAg- -3' miRNA: 3'- gGUCAuuaa---CGaUGUUGUUGUCGUag -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 45409 | 0.68 | 0.999973 |
Target: 5'- uCCAGcauugcaucgGCUugGGCGGCcGCAUCg -3' miRNA: 3'- -GGUCauuaa-----CGAugUUGUUGuCGUAG- -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 120656 | 0.68 | 0.99997 |
Target: 5'- cUCGGaGGUcUGCaugUACAugAACGGCGUCg -3' miRNA: 3'- -GGUCaUUA-ACG---AUGUugUUGUCGUAG- -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 37342 | 0.68 | 0.999958 |
Target: 5'- cCCAGggcgcuuGUUGgUGCGGCGGCGG-AUCg -3' miRNA: 3'- -GGUCau-----UAACgAUGUUGUUGUCgUAG- -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 66709 | 0.69 | 0.999943 |
Target: 5'- cUCGGUagcGAUUGCaccgUACAACAAgAGCAa- -3' miRNA: 3'- -GGUCA---UUAACG----AUGUUGUUgUCGUag -5' |
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3512 | 5' | -43.6 | NC_001623.1 | + | 64102 | 0.69 | 0.999898 |
Target: 5'- gCAGUAAccuaUACAAUAGCGGCGUg -3' miRNA: 3'- gGUCAUUaacgAUGUUGUUGUCGUAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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